Controlled release of TGF-beta 1 from RADA self-assembling peptide hydrogel scaffolds.
Controlled release of TGF-beta 1 from RADA self-assembling peptide hydrogel scaffolds.
复制标题
RADA 自组装肽水凝胶支架中受控释放 TGF-β1
DOI：
10.2147/dddt.s109545
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Jiang D
中科院分区：
文献类型：
--
作者：
Zhou A;Chen S;He B;Zhao W;Chen X;Jiang D
生物活性介质、细胞因子和趋化因子在调节和优化组织工程中材料、细胞和细胞微环境的协同作用方面发挥着重要作用。RADA自组装肽水凝胶已被证明具有良好的促进细胞增殖、伤口愈合、组织修复和药物输送的能力。在这里，我们报道了D-RADA16和L-RADA16-RGD自组装肽可以形成稳定的二级结构和水凝胶支架，提供生长因子(转化生长因子细胞因子-β1)的缓慢释放。体外释放试验表明，该制剂可在72小时内达到平台释放量。将含转化生长因子-β1的L-RADA16、D-RADA16和L-RADA16-RGD自组装多肽水凝胶用于大鼠骨髓间充质干细胞三维培养2周。结果表明，这三种RADA16多肽水凝胶对骨髓间充质干细胞的增殖有明显的促进作用，对生长因子的缓释有一定的应用前景。
Bioactive mediators, cytokines, and chemokines have an important role in regulating and optimizing the synergistic action of materials, cells, and cellular microenvironments for tissue engineering. RADA self-assembling peptide hydrogels have been proved to have an excellent ability to promote cell proliferation, wound healing, tissue repair, and drug delivery. Here, we report that D-RADA16 and L-RADA16-RGD self-assembling peptides can form stable second structure and hydrogel scaffolds, affording the slow release of growth factor (transforming growth factor cytokine-beta 1 [TGF-beta 1]). In vitro tests demonstrated that the plateau release amount can be obtained till 72 hours. Moreover, L-RADA16, D-RADA16, and L-RADA16-RGD self-assembling peptide hydrogels containing TGF-beta 1 were used for 3D cell culture of bone mesenchymal stem cells of rats for 2 weeks. The results revealed that these three RADA16 peptide hydrogels had a significantly favorable influence on proliferation of bone mesenchymal stem cells and hold some promise in slow and sustained release of growth factor.