Cellular responses to chemical and morphologic aspects of biomaterial surfaces. I. A novel in vitro model system.
Cellular responses to chemical and morphologic aspects of biomaterial surfaces. I. A novel in vitro model system.
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DOI：
10.1002/jbm.820290909
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发表时间：
1995-09
期刊：
影响因子：
--
通讯作者：
K. Chesmel;Jonathan Black
中科院分区：
文献类型：
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作者：
K. Chesmel;Jonathan Black
任何植入物的临床成功直接取决于宿主组织和用于制造器械的生物材料之间建立的界面附近的细胞行为。所有生物材料都具有影响细胞对植入物反应的形态学、化学和电表面特征。定量测量这种局部宿主对不同但表征良好的生物材料表面的反应的特定方面，为理解植入物生物相容性的整体现象提供了至关重要的联系。一个系统已被设计用于在体外检查细胞的反应控制，但独立的变化，在聚苯乙烯（PS）组织培养表面的化学和形态。使用微机械加工的硅晶片作为模板以溶剂浇铸PS复制品[使用0、1或2wt%苯乙烯（S）单体添加物]，其中无、0.5或5.0微米深的表面凹槽以径向阵列排列。当所有可能的形态与所有可能的聚合物相结合时，产生了九种模型生物材料表面（MBS）。的MBS的化学特性进行了测定，使用电子能谱化学分析，二次离子质谱，和接触角技术，并被发现是不同的。检查了从含血清培养基吸附到这些表面上的蛋白质的类型和量，发现其由相对均匀组成的多个分子层组成。由MBS形成的自含式组织培养容器能够支持大鼠颅骨细胞的汇合培养物的生长。这里描述的模型生物材料系统可用于检查由测试材料的化学和形态特征引起的同时刺激如何影响生物反应。需要进行这种多因素生物相容性研究，以适当记录材料-宿主相互作用。
The clinical success of any implant is directly dependent upon the cellular behavior in the immediate vicinity of the interface established between the host tissue and the biomaterial(s) used to fabricate the device. All biomaterials have morphologic, chemical, and electrical surface characteristics that influence the cellular response to the implant. Quantitative measurement of specific aspects of this local host response to different but well-characterized biomaterial surfaces provides a crucial link in the understanding of the overall phenomenon of implant biocompatibility. A system has been devised for in vitro examination of responses of cells to controlled but independent changes in both the chemistry and morphology of polystyrene (PS) tissue culture surfaces. Micromachined silicon wafers were used as templates to solvent-cast PS replicas [using 0, 1, or 2 wt % styrene (S) monomer additions] with either none, 0.5- or 5.0-microns-deep surface grooves arranged in a radial array. When all possible morphologies were combined with all possible polymers, nine model biomaterial surfaces (MBSs) were produced. The chemical characteristics of the MBSs were determined using electron spectroscopy for chemical analysis, secondary ion mass spectroscopy, and contact angle techniques and were found to be distinct. The types and amount of proteins that adsorb onto these surfaces from serum containing media were examined and found to consist of multiple molecular layers of relatively uniform composition. Self-contained tissue culture vessels formed from the MBSs were capable of supporting the growth of confluent cultures of rat calvarial cells. The model biomaterial system described here can be used to examine how simultaneous stimuli resulting from the chemical and morphological characteristics of a test material may influence biologic responses. Such multifactorial biocompatibility research is needed to properly document material-host interactions.