Barcoding bacterial cells: A SERS based methodology for pathogen identification.
Barcoding bacterial cells: A SERS based methodology for pathogen identification.
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DOI：
10.1002/jrs.2064
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发表时间：
2008-11
影响因子：
2.5
通讯作者：
Ziegler, L. D.
中科院分区：
文献类型：
--
作者：
Patel, I. S.;Premasiri, W. R.;Moir, D. T.;Ziegler, L. D.
A principal component analysis (PCA) based on the sign of the second derivative of the surface enhanced Raman spectroscopy (SERS) spectrum obtained on in-situ grown Au cluster covered SiO2 substrates results in improved reproducibility and enhanced specificity for bacterial diagnostics. The barcode generated clustering results are systematically compared to those obtained from corresponding spectral intensities, first derivatives and second derivatives for the SERS spectra of closely related cereus group Bacillus strains. PCA plots and corresponding hierarchical cluster analysis (HCA) dendrograms illustrate the improved bacterial identification resulting from the barcode spectral data reduction. Supervised DFA plots result in slightly improved group separation but show more susceptibility to false positive classifications than the corresponding PCA contours. In addition, this PCA treatment is used to highlight the enhanced bacterial species specificity observed for SERS as compared to normal bulk (non-SERS) Raman spectra. The identification algorithm described here is critical for the development of SERS microscopy as a rapid, reagentless, portable diagnostic of bacterial pathogens.
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影响因子：
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通讯作者：
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