Ribonuclease inhibitor from human placenta: rapid purification and assay.
Ribonuclease inhibitor from human placenta: rapid purification and assay.
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发表时间：
1979-12
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通讯作者：
P. Blackburn
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作者：
P. Blackburn
两天内即可从人胎盘中分离出核糖核酸酶抑制剂，收率可达 65%（每个胎盘 2.5 毫克）。通过采用核糖核酸酶对 2',3'-环胞苷单磷酸的分光光度测定来测定抑制剂活性，加快了 Sepharose-RNase A 上亲和层析的性能。这些程序改进的结果是增加了核糖核酸酶抑制剂的可用性，用于研究其化学性质（Blackburn，P.和Jailkhani，B.L.（1979）J.Biol.Chem.254，12488-12493）以及将其纳入需要抑制哺乳动物中性核糖核酸酶的体外系统中。
The ribonuclease inhibitor from human placenta may be isolated in 65% yield (2.5 mg per placenta) in 2 days. The performance of the affinity chromatography on Sepharose-RNase A has been expedited through adaption of the spectrophotometric assay of ribonuclease toward 2',3'-cyclic cytidine monophosphate to determination of the inhibitor activity. The result of these improvements in procedure is increased availability of the ribonuclease inhibitor for study of its chemical properties (Blackburn, P., and Jailkhani, B.L. (1979) J. Biol. Chem. 254, 12488-12493) and for its inclusion into in vitro systems in which inhibition of mammalian neutral ribonucleases is desired.