Rhodopsin-EGFP knock-ins for imaging quantal gene alterations
Rhodopsin-EGFP knock-ins for imaging quantal gene alterations
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DOI：
10.1016/j.visres.2005.07.016
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发表时间：
2005-12-01
期刊：
影响因子：
1.8
通讯作者：
Wilson, JH
中科院分区：
文献类型：
--
作者：
Wensel, TG;Gross, AK;Wilson, JH
我们已经开发了一种成像方法来监测光感受器基因结构的变化。我们在此回顾战略和最近的进展。携带人类视紫红质- egfp融合基因的敲入小鼠可能允许检测单个分子事件：在整个视网膜内纠正一个基因的单个拷贝。利用共聚焦或多光子荧光成像技术，这些小鼠也可用于成像正常小鼠或疾病状态下的视紫红质分布、膜结构和运输。它们是研究光感受器发育的分子触发、跟踪干细胞群体和评估视网膜移植实验的工具。(c) 2005 Elsevier Ltd版权所有。
We have developed an imaging approach to monitor changes in gene structure in photoreceptors. We review here, the strategy and recent progress. Knock-in mice bearing a human rhodopsin-EGFP fusion gene potentially allow detection of a single molecular event: correction of a single copy of a gene within an entire retina. These mice can also be used for imaging rhodopsin distribution, membrane structure, and trafficking in normal mice or in disease states, using confocal or multiphoton fluorescence imaging techniques. They represent tools for studying molecular triggers of photoreceptor development, for following stem cell populations, and for evaluating retinal transplantation experiments. (c) 2005 Elsevier Ltd. All rights reserved.