MAPPING OF INVIVO MESSENGER-RNAS FOR BACTERIOPHAGE-PHIX-174
MAPPING OF INVIVO MESSENGER-RNAS FOR BACTERIOPHAGE-PHIX-174
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DOI：
10.1073/pnas.73.10.3519
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发表时间：
1976-01-01
影响因子：
11.1
通讯作者：
HAYASHI, M
中科院分区：
文献类型：
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作者：
HAYASHI, M;FUJIMURA, FK;HAYASHI, M
在琼脂糖凝胶中将v φ X-174噬菌体的体内mRNA分级分离成许多大小范围为0.23 × 10 - 6的离散种类。106-2.3倍106道尔顿。将这些RNA物质从凝胶上洗脱下来，并通过用[Hind II、Hap II、Hpa I和Hin H-I]限制性酶[内切核酸酶]切割而与源自v φ X-174复制型DNA的特异性片段杂交。构建了体内mRNA相对于噬菌体遗传图谱的方向图。该图谱表明，mRNA的起始发生在基因B、基因C或D之前，并且可能发生在基因A之前，终止发生在基因E、F、G和H之后。在任何特定位点终止转录可能不是完全有效的，从而观察到许多具有相同5“-末端的重叠转录物。基因A的mRNA可能相对不稳定。
In vivo mRNA for bacteriophage .vphi.X-174 was fractionated in agarose gels into a number of discrete species ranging in size from 0.23 .times. 106-2.3 .times. 106 daltons. These RNA species were eluted from the gels and hybridized to specific fragments derived from .vphi.X-174 replicative form DNA by cleavage with [Hind II, Hap II, Hpa I and Hin H-I] restriction enzymes [endonucleases]. A map of the orientation of in vivo mRNAs with respect to the bacteriophage genetic map was constructed. This map indicated that initiation of mRNA occurred before gene B, gene C or D, and probably before gene A and that termination occurred after genes E, F, G and H. Termination of transcription at any particular site may not to be entirely effective such that a number of overlapping transcripts with the same 5''-terminus was observed. mRNA for gene A may be relatively unstable.