Histogenesis of mouse cerebellum in microwell cultures. Cell reaggregation and migration, fiber and synapse formation
Histogenesis of mouse cerebellum in microwell cultures. Cell reaggregation and migration, fiber and synapse formation
复制标题
微孔培养物中小鼠小脑的组织发生。
作者：
E. Trenkner;R. Sidman
开发了微孔培养系统，用于分析神经系统组织发生期间的细胞运动和相互作用。来自胰蛋白酶处理的7天龄小鼠小脑的细胞在数小时内重新聚集成簇，这些簇后来发展出由迁移细胞和细胞突起的片材或纤维束与沿着其表面迁移的细胞的电缆组成的互连。颗粒细胞在几个阶段的分化，篮状和/或星状神经元，一些较大的神经元，和两种类型的神经胶质细胞进行了鉴定，在可重复的，非随机模式的扫描和透射电子显微镜。轴突和树突的过程，都与生长锥，和许多突触在体外产生。
A microwell culture system was developed for analysis of cell movements and interactions during nervous system histogenesis. Cells from trypsinized 7-day-old mouse cerebellum reaggregated within hours into clusters which later developed interconnections consisting of either sheets of migrating cells and cell processes or cables of fiber bundles with cells migrating along their surfaces. Granule cells in several stages of differentiation, basket and/or stellate neurons, some larger neurons, and two types of neuroglial cells were identified in reproducible, nonrandom patterns by scanning and transmission electron microscopy. Axonal and dendritic processes, both with growth cones, and numerous synapses were generated in vitro.