Induction of DNA double-strand breaks by 1H and 4He ions in primary human skin fibroblasts in the LET range of 8 to 124 keV/μm
Induction of DNA double-strand breaks by 1H and 4He ions in primary human skin fibroblasts in the LET range of 8 to 124 keV/μm
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DOI：
10.2307/3580030
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发表时间：
1999-05-01
影响因子：
3.4
通讯作者：
Pralle, E
中科院分区：
文献类型：
--
作者：
Frankenberg, D;Brede, HJ;Pralle, E
DNA双链断裂的产量在原代人皮肤成纤维细胞暴露于H-1和He-4离子在各种线性能量转移（LET）和15 MeV的电子作为参考辐射。在LET为35，23和7.9千电子伏/亩米，分别为H-1离子和1.2，1.18，1.38和1.31为He-4离子在LET为124，76，35和27千电子伏/亩米，分别为2.03，1.45和1.36。使用脉冲场凝胶电泳的DNA从细胞中释放的酵母酿酒酵母的染色体作为长度标记，并拟合的实验质量分布的片段化的DNA的随机断裂的人类染色体的计算机模拟获得的数据。在哺乳动物细胞中诱导DSB的RBE值不能适合于电子和H-1，He-4和轻离子的常见RBE-LET关系。哺乳动物细胞的RBE与酵母细胞获得的相应RBE的比较显示，酵母和哺乳动物细胞的电子的RBE相似;然而，对于He-4和轻离子在100至1000 keV/μ m的LET范围内，酵母的RBE显著高于哺乳动物细胞。酵母和哺乳动物细胞的RBE-LET关系的这些特征归因于当穿过在这两种细胞类型中在一定程度上不同的高阶染色质结构时由颗粒诱导的小DNA片段的分数。(C)1999年，辐射研究协会。
Yields of DNA double-strand breaks were determined in primary human skin fibroblasts exposed to H-1 and He-4 ions at various linear energy transfers (LETs) and to 15 MeV electrons as the reference radiation. The values obtained for the relative biological effectiveness (RBE) were 2.03, 1.45 and 1.36 for H-1 ions at LETs of 35, 23 and 7.9 keV/mu m, respectively, and 1.2, 1.18, 1.38 and 1.31 for He-4 ions at LETs of 124, 76, 35 and 27 keV/mu m, respectively. The data were obtained using pulsed-field gel electrophoresis of DNA released from cells using the chromosomes of the yeast Saccharomyces ces cerevisiae as length markers and fitting the experimental mass distributions of fragmented DNA to those obtained by computer simulation of the random breakage of human chromosomes. The RBE values for induction of DSBs in mammalian cells cannot be fitted to a common RBE-LET relationship for electrons and H-1, He-4 and light ions. Comparison of the RBEs for mammalian cells with the corresponding RBEs obtained for yeast cells shows similar RBEs of electrons for yeast and mammalian cells; however, for He-4 and light ions in the LET range of 100 to 1000 keV/mu m, the RBEs for yeast are significantly higher compared with mammalian cells. These characteristics of the RBE-LET relationships for yeast and mammalian cells are attributed to the fraction of small DNA fragments induced by particles when traversing the higher-order chromatin structures which are different to some extent in these two cell types. (C) 1999 by Radiation Research Society.