FIRING PROPERTIES OF SUBSTANTIA NIGRA DOPAMINERGIC-NEURONS IN FREELY MOVING RATS
FIRING PROPERTIES OF SUBSTANTIA NIGRA DOPAMINERGIC-NEURONS IN FREELY MOVING RATS
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DOI：
10.1016/0024-3205(85)90448-5
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发表时间：
1985-01-01
期刊：
影响因子：
6.1
通讯作者：
BUNNEY, BS
中科院分区：
文献类型：
--
作者：
FREEMAN, AS;MELTZER, LT;BUNNEY, BS
从清醒、自由活动的大鼠的黑质中推测的多巴胺能神经元中获得单单位记录。这些细胞表现出的波形、放电速率范围和放电模式类型与麻醉或瘫痪大鼠的DA神经元相同。观察到两种放电模式：单尖峰活动和脉冲模式，每次脉冲中尖峰的振幅逐渐减弱，持续时间增加。细胞间的突发放电程度差异很大，单个细胞有时会从一种放电模式（例如，主要是单尖峰）切换到另一种模式，例如，突发，尽管这些转变的决定因素目前尚不清楚。假定的DA神经元受到阿波啡的抑制，氟哌啶醇的兴奋。氟哌啶醇也逆转了阿吗啡诱导的放电抑制。抑制和兴奋分别与突发射击的降低和升高有关。这两种药物的作用与它们在固定大鼠中的作用相同。在几个案例中，我们观察到一个假定的DA神经元与至少一个具有相同电生理特征的其他细胞几乎同时发射其所有的尖峰。这种DA细胞之间的相互作用（即假定的电耦合）仅在麻醉或瘫痪大鼠中很少观察到，并且可能在黑质纹状体DA系统的正常功能中发挥重要作用。
Single unit recordings were obtained from putative dopaminergic neurons in the substantia nigra of awake, freely moving rats. The cells exhibited waveforms, range of firing rates and types of firing patterns identical to those of identified DA neurons of anesthetized or paralyzed rats. Two firing patterns were observed: single spike activity and a bursting mode with spikes of progressively diminished amplitude and increased duration within each burst. The degree of burst firing varied considerably among the cells and individual cells sometimes switched from one pattern of firing (e.g., predominantly single spike) to another, e.g., bursting, although the determinants of these transitions are, at this time, unclear. Putative DA neurons were inhibited by i.v. apomorphine and excited by i.v. haloperidol. Haloperidol also reversed the apomorphine-induced inhibition of firing. Inhibitions and excitations were associated with a reduction and elevation, respectively, in burst firing. The effects of the 2 drugs were identical to their effects in immobilized rats. In several cases, a putative DA neuron was observed to fire all of its spikes in near coincidence with at least 1 other cell with identical electrophysiological characteristics. This form of interaction (i.e., presumed electrical coupling) between DA cells is only rarely observed in anesthetized or paralyzed rats and may play a significant role in the normal functioning of the nigrostriatal DA system.