Induced sputum cell counts in healthy adults
Induced sputum cell counts in healthy adults
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DOI：
10.1164/ajrccm.161.2.9903097
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发表时间：
2000-02-01
影响因子：
24.7
通讯作者：
Hargreave, FE
中科院分区：
文献类型：
--
作者：
Belda, J;Leigh, R;Hargreave, FE
诱导痰细胞计数提供了一种相对无创的方法来评估肺部气道炎症的存在、类型和程度。它们的解释需要了解健康人群的正常值。目的是检查健康成人样本诱导痰中的总细胞计数和分类细胞计数。共有118名健康的非吸烟成年人参加了研究。无哮喘或气流阻塞（阴性病史，FEV 1大于或等于80%预测值，FEV 1/肺活量比值[FEV 1/VC]大于或等于80%，乙酰甲胆碱PC 20大于或等于16 mg/ml）。46例为特应性。痰诱导在96名受试者中产生了足够的样本[53名男性，平均年龄（范围）36（18至60）岁]。在2小时内处理痰液;选择痰液，用二硫苏糖醇处理，过滤，并在血细胞计数器中检查总细胞计数和活力，并在瑞氏染色的细胞离心涂片上进行细胞分类计数。平均、中位（第90百分位数）总细胞计数为4.1，2.4（9.7）× 10（6）个细胞/g，细胞活力为69.6，72.0（89.7）%。嗜酸性粒细胞的比例分别为0.4、0.0（1.1）%，中性粒细胞37.5、36.7（64.0）%，巨噬细胞58.8、60.8（86.1）%，淋巴细胞1.0、0.5（2.6）%，异染性细胞0.0、0.0（0.04）%，支气管上皮细胞1.6、0.3（4.4）%。女性和特应性与嗜酸性粒细胞显著升高相关;男性/女性之间的平均差异为0.3%（p = 0.043），特应性/非特应性之间的平均差异为0.4%（p = 0.024）。本研究确定了健康成人诱导痰中细胞总数和分类计数的参考值。
Induced sputum cell counts provide a relatively noninvasive method to evaluate the presence, type, and degree of inflammation in the airways of the lungs. Their interpretation requires a knowledge of normal values from a healthy population. The objective was to examine the total and differential cell counts in induced sputum from a sample of healthy adults. A total of 118 healthy nonsmoking adults were studied. None had asthma or airflow obstruction (negative history, FEV1 greater than or equal to 80% predicted, ratio of FEV1 to vital capacity [FEV1/VC] greater than or equal to 80%, methacholine PC20 greater than or equal to 16 mg/ml). Forty-six were atopic. Sputum induction produced an adequate sample in 96 subjects [53 males, mean age (range) 36 (18 to 60) yr]. The expectorate was processed within 2 h; sputum was selected, treated with dithiothreitol, filtered, and examined in a hemocytometer for total cell count and viability and on Wright-stained cytospins for a differential cell count. The mean, median (90th percentile) total cell count was 4.1, 2.4 (9.7) x 10(6) cells/g and cell viability was 69.6, 72.0 (89.7)%. The proportions of eosinophils were 0.4, 0.0 (1.1)%, neutrophils 37.5, 36.7 (64.0)%, macrophages 58.8, 60.8 (86.1)%, lymphocytes 1.0, 0.5 (2.6)%, metachromatic cells 0.0, 0.0 (0.04)%, and bronchial epithelial cells 1.6, 0.3 (4.4)%, respectively. Female gender and atopy were associated with a significant elevation of eosinophils; mean difference between male/female was 0.3% (p = 0.043) and between atopic/nonatopic 0.4% (p = 0.024). This study has identified reference values for total and differential cell counts in induced sputum of healthy adults.