Two subunits of the Drosophila mediator complex act together to control cell affinity
Two subunits of the Drosophila mediator complex act together to control cell affinity
复制标题
DOI：
10.1242/dev.00607
复制
发表时间：
2003-08-01
期刊：
影响因子：
4.6
通讯作者：
Treisman, JE
中科院分区：
文献类型：
--
作者：
Janody, F;Martirosyan, Z;Treisman, JE
果蝇想象圆盘发育的组织中心位于隔室之间的直线边界上；这些是由选择基因和细胞间信号控制的细胞亲和力差异维持的。skld和kohtalo编码果蝇中介复合物的两个最大亚基TRAP240和TRAP230的同源物；任何一个基因的突变都会导致相同的表型。我们在这里表明，这两个基因都需要在翅盘的前后和背腹隔室边界建立正常的细胞亲和力差异。突变细胞从前室穿过后室，可以在背侧或腹侧方向扭曲背-腹侧边界。Skuld和Kohtalo蛋白在体内相互作用，并在过表达时具有协同效应，与Skuld Kohtalo双突变表型一致，与单突变型无法区分。我们认为这两个亚基不参与介质复合体的所有活动，但形成一个需要调节特定靶基因的子模块，包括那些控制细胞亲和力的基因。
The organizing centers for Drosophila imaginal disc development are created at straight boundaries between compartments; these are maintained by differences in cell affinity controlled by selector genes and intercellular signals. skuld and kohtalo encode homologs of TRAP240 and TRAP230, the two largest subunits of the Drosophila mediator complex; mutations in either gene cause identical phenotypes. We show here that both genes are required to establish normal cell affinity differences at the anterior-posterior and dorsal-ventral compartment boundaries of the wing disc. Mutant cells cross from the anterior to the posterior compartment, and can distort the dorsal-ventral boundary in either the dorsal or ventral direction. The Skuld and Kohtalo proteins physically interact in vivo and have synergistic effects when overexpressed, consistent with a skuld kohtalo double-mutant phenotype that is indistinguishable from either single mutant. We suggest that these two subunits do not participate in all of the activities of the mediator complex, but form a submodule that is required to regulate specific target genes, including those that control cell affinity.