Molecular genetic mapping of quantitative trait loci for milling quality in rice (Oryza sativa L.)
Molecular genetic mapping of quantitative trait loci for milling quality in rice (Oryza sativa L.)
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DOI：
10.1016/j.jcs.2004.04.008
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发表时间：
2004-09-01
影响因子：
3.8
通讯作者：
Cheng, SH
中科院分区：
文献类型：
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作者：
Dong, YJ;Tsuzuki, E;Cheng, SH
为选育优质稻米新品种，通过对水稻碾磨性状的QTLs定位，确定了与碾磨性状相关的基因组区域。所用的重组自交系（RI）群体是通过289个RFLP标记由粳稻品种‘Asominori’与籼稻品种‘IR 24’杂交获得的。对各RI系及其亲本的糙米率（BRP）、精米率（MRP）和整精米率（MHP）这3个稻米加工品质的主要指标进行了测定。3个碾磨性状在RI群体中呈连续分布和超亲分离，表明这3个性状均为数量遗传。在第9和第10染色体上分别定位了2个BRP QTL（qBRP-9和qBRP-10），分别解释了7.2%和21.3%的表型变异。检测到2个控制MRP的QTL（qMRP-11和qMRP-12），分别位于第11和12染色体上，占总表型变异的12.2%和7.7%。此外，还检测到3个控制MHP的QTL（qMHP-1、qMHP-3和qMHP-5），分别定位在第1、3和5染色体上，分别解释了总表型变异的16.0%、22.1%和8.7%。其中，来自粳稻亲本Asominori的qBRP-9、qBRP-10、qMRP-11、qMHP-3和qMHP-5等5个QTL。来自籼稻1824的2个QTL（qMRP-12、qMHP-1）可以提高稻米碾磨品质。本研究的结果和该QTL侧翼的紧密连锁分子标记将为稻米碾磨品质的改良育种提供参考。(C)2004爱思唯尔有限公司保留所有权利。
Identification and mapping of genomic regions controlling quantitative trait loci (QTLs) was undertaken to determine the genomic regions associated with milling traits in rice to facilitate breeding of new rice varieties with high milling quality. The recombinant inbred (RI) population used was derived from cross of a japonica variety, `Asominori', with an indica variety, 'IR24' through 289 RFLP markers. Three milling traits, namely, brown rice percentage (BRP), milled rice percentage (MRP), and milled head rice percentage (MHP), which are the main indicators of milling quality in rice, were estimated for each RI line and their parental varieties. Continuous distributions and transgressive segregations of three milling traits were observed in the RI population, showing that the three traits were quantitatively inherited. Two QTLs (qBRP-9 and qBRP-10) for BRP were identified and mapped to chromosomes 9 and 10, and explained 7.2 and 21.3% of the total phenotype variation, respectively. Two QTLs (qMRP-11 and qMRP-12) governing MRP were detected and mapped to chromosomes 11 and 12, accounted for 12.2 and 7.7% of total phenotype variation, respectively. In addition, three QTLs (qMHP-1, qMHP-3 and qMHP-5) controlling MHP were observed and mapped to chromosomes 1, 3 and 5, and explained 16.0, 22.1 and 8.7% of the total phenotype variation, respectively. Among them, five QTLs (qBRP-9, qBRP-10, qMRP-11, qMHP-3 and qMHP-5) from japonica parent, Asominori. and two QTLs (qMRP-12, qMHP-1) from indica 1824 can improve milling quality in rice. The results and the tightly linked molecular markers that flank the QTL will be useful in breeding for improvement of milling quality in rice. (C) 2004 Elsevier Ltd. All rights reserved.