Single-cell transcriptomic analysis of vascular endothelial cells in zebrafish embryos.
Single-cell transcriptomic analysis of vascular endothelial cells in zebrafish embryos.
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DOI：
10.1038/s41598-022-17127-w
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发表时间：
2022-07-29
影响因子：
4.6
通讯作者：
Sumanas, Saulius
中科院分区：
文献类型：
--
作者：
Gurung, Suman;Restrepo, Nicole K.;Chestnut, Brendan;Klimkaite, Laurita;Sumanas, Saulius
血管内皮细胞表现出大量的表型和转录异质性，这是建立在早期胚胎发生。然而，参与建立内皮细胞多样性的分子机制仍然没有得到很好的理解。斑马鱼已成为研究血管发育的有利模型。尽管其重要性，血管内皮细胞在斑马鱼发育过程中的单细胞转录组学特征仍然缺失。为了解决这个问题，我们应用了从24 hpf阶段的斑马鱼胚胎中分离的血管内皮细胞的单细胞RNA测序（scRNA-seq）。鉴定了与血管内皮细胞相关的六个不同的簇或亚簇，其包括动脉、两个静脉、颅、内皮和内皮祖细胞亚型。此外，我们通过表征动脉、静脉和内皮细胞的新标志物来验证我们的发现。我们通过实验证实了存在两种转录上不同的静脉细胞亚型，证明了静脉内皮细胞在早期发育阶段的异质性。这个数据集将是一个宝贵的资源，为未来的功能表征血管内皮细胞和审问的分子机制参与建立其异质性和细胞命运的决定。
Vascular endothelial cells exhibit substantial phenotypic and transcriptional heterogeneity which is established during early embryogenesis. However, the molecular mechanisms involved in establishing endothelial cell diversity are still not well understood. Zebrafish has emerged as an advantageous model to study vascular development. Despite its importance, the single-cell transcriptomic profile of vascular endothelial cells during zebrafish development is still missing. To address this, we applied single-cell RNA-sequencing (scRNA-seq) of vascular endothelial cells isolated from zebrafish embryos at the 24 hpf stage. Six distinct clusters or subclusters related to vascular endothelial cells were identified which include arterial, two venous, cranial, endocardial and endothelial progenitor cell subtypes. Furthermore, we validated our findings by characterizing novel markers for arterial, venous, and endocardial cells. We experimentally confirmed the presence of two transcriptionally different venous cell subtypes, demonstrating heterogeneity among venous endothelial cells at this early developmental stage. This dataset will be a valuable resource for future functional characterization of vascular endothelial cells and interrogation of molecular mechanisms involved in the establishment of their heterogeneity and cell-fate decisions.
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DOI：
10.1126/science.1247651
发表时间：
2014-02-14
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Jaitin DA;Kenigsberg E;Keren-Shaul H;Elefant N;Paul F;Zaretsky I;Mildner A;Cohen N;Jung S;Tanay A;Amit I
通讯作者：
Amit I
影响因子：
64.8
作者：
Cao, Junyue;Spielmann, Malte;Shendure, Jay
通讯作者：
Shendure, Jay
影响因子：
4.8
作者：
Bayless, KJ;Davis, GE
通讯作者：
Davis, GE
影响因子：
2.5
作者：
Chestnut, Brendan;Sumanas, Saulius
通讯作者：
Sumanas, Saulius
DOI：
10.1002/dvg.23021
发表时间：
2017-03
期刊：
Genesis (New York, N.Y. : 2000)
影响因子：
--
作者：
Iklé JM;Tavares AL;King M;Ding H;Colombo S;Firulli BA;Firulli AB;Targoff KL;Yelon D;Clouthier DE
通讯作者：
Clouthier DE