A comparison of GABA- and GAD-like immunoreactivity within the area postrema of the rat and cat.
A comparison of GABA- and GAD-like immunoreactivity within the area postrema of the rat and cat.
复制标题
大鼠和猫后区 GABA 和 GAD 样免疫反应性的比较。
DOI：
10.1002/cne.902550205
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Maley,BE
中科院分区：
文献类型：
--
作者：
Newton,BW;Maley,BE
借助兔中产生的抗GABA多克隆抗体，比较大鼠和猫最后区中γ-氨基丁酸（GABA）与谷氨酸脱羧酶（GAD）的免疫组织化学定位。在大鼠和猫中，GABA样免疫反应性（GABA-LI）静脉曲张的密集和极密集积聚遍布最后区。GABA-LI细胞体存在于两个物种中，并且均匀分布在整个最后区范围内。然而，大鼠最后区比猫最后区含有更多的GABA-LI细胞体和静脉曲张。在猫最后区，用GABA抗体对一系列细胞大小进行免疫染色。然而，GAD抗体未能显示猫最后区的细胞体，因此表明GABA多克隆抗体可能是含GABA胞体的更好指示剂。虽然GABA在最后区的作用机制尚不清楚，但GABA可能在呕吐和非呕吐物种的最后区的不同功能中发挥作用。
The immunohistochemical localization of gamma‐aminobutyric acid (GABA) was compared to that of glutamate decarboxylase (GAD) in the rat and cat area postrema with the aid of a polyclonal antibody produced in rabbits directed against GABA. In both the rat and cat, dense and very dense accumulations of GABA‐like immunoreactive (GABA‐LI) varicosities were present throughout the area postrema. GABA‐LI cell bodies were present in both species and were evenly distributed throughout the area postrema's extent. However, the rat area postrema contained more GABA‐LI cell bodies and varicosities than the cat area postrema. In the cat area postrema, a range of cell sizes were immunostained with the GABA antibody. The GAD antibody, however, failed to reveal cell bodies in the area postrema of the cat, thus indicating that the GABA polyclonal antibody may be a better indicator of GABA‐containing somata. Although the mechanism of action of GABA in the area postrema is not understood, it is possible that GABA may play a role in the different functions of the area postrema in emetic and nonemetic species.