Identification of long noncoding RNAs of silkworm at the early stage of Bombyx mori bidensovirus infection
Identification of long noncoding RNAs of silkworm at the early stage of Bombyx mori bidensovirus infection
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DOI：
10.1002/arch.22082
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发表时间：
2024-01-01
影响因子：
2.2
通讯作者：
Zhang，Zhendong
中科院分区：
文献类型：
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作者：
Chen，Yeping;Wang，Zihe;Zhang，Zhendong
家蚕比登索病毒(BmBDV)是家蚕最重要的病原之一。主要侵染家蚕中肠细胞，给蚕业造成损失。据报道，长非编码RNA(LncRNAs)在家蚕抗病毒免疫反应的调节中发挥重要作用。为了探讨lncRNAs是否参与家蚕对BmBDV的感染和免疫应答，我们对感染BmBDV的家蚕幼虫和未感染BmBDV的家蚕幼虫进行了比较转录组学分析，鉴定了早期感染BmBDV的家蚕幼虫的lncRNAs和mRNAs。共鉴定出16069个基因和974个候选LncRNAs，其中142个信使RNA(MRNAs)和4个LncRNAs差异表达(DE)。靶基因预测结果显示，142个DEmRNAs与4个DElncRNAs共表达，提示该基因的表达主要通过反式调节活性来实现。构建了一个由DElncRNAs和DEmRNAs组成的调控网络，表明不同的DElncRNAs靶向的许多基因参与了代谢和免疫，这意味着这些基因和lncRNAs在BmBDV的复制中起着重要的作用。我们的结果将有助于我们更好地理解lncRNA在BmBDV感染中的调节作用，为进一步探索宿主与BmBDV之间的相互作用提供新的视角。
Bombyx mori bidensovirus(BmBDV) is one of the most important pathogens of silkworm. It mainly infects midgut cells of silkworm and causes losses to the sericulture industry. Long noncoding RNAs (lncRNAs) have been reported to play an important role in the regulation of antiviral immune response in silkworm. To explore whether lncRNAs are involved in BmBDV infection and immune response of silkworm, we performed a comparative transcriptome analysis to identify the lncRNAs and mRNAs between the BmBDV infected and noninfected silkworm larvae at the early stage. A total of 16,069 genes and 974 candidate lncRNAs were identified, among which 142 messenger RNA (mRNAs) and four lncRNAs were differentially expressed (DE). Target gene prediction revealed that 142 DEmRNAs were coexpressed with four DElncRNAs, suggesting that the expression of mRNA is mainly affected throughtrans‐regulation activities. A regulatory network of DElncRNAs and DEmRNAs was constructed, showing that many genes targeted by different DElncRNAs are involved in metabolism and immunity, which implies that these genes and lncRNAs play an important role in the replication of BmBDV. Our results will help us to improve our understanding of lncRNA‐mediated regulatory roles in BmBDV infection, providing a new perspective for further exploring the interaction between host and BmBDV.