Two host microRNAs influence WSSV replication via STAT gene regulation.
Two host microRNAs influence WSSV replication via STAT gene regulation.
复制标题
两种宿主 microRNA 通过 STAT 基因调控影响 WSSV 复制
DOI：
10.1038/srep23643
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发表时间：
2016-03-31
影响因子：
4.6
通讯作者：
Ren Q
中科院分区：
文献类型：
--
作者：
Huang Y;Wang W;Ren Q
MicroRNAs(MiRNAs)在基因表达的转录后调控中发挥着重要作用。在病毒感染期间，病毒利用宿主通过改变细胞miRNAs来增强复制。Janus激酶(JAK)/信号转导和转录激活因子(STAT)通路在抗病毒应答中起着关键作用。本研究发现罗氏沼虾的两个miRNAs(miR-9041和miR-9850)能促进白斑综合症病毒(WSSV)的复制。MiR-9041或miR-9850的上调抑制了罗氏沼虾鳃中STAT的表达，从而下调了其下游动力蛋白(Dnm)基因的表达：DNM1、Dnm2和Dnm3。MiR-9041和miR-9850的敲除通过上调STAT和Dnm基因的表达来限制WSSV的复制。STAT、DNM1、DNM2或DNM3的沉默导致对虾中WSSV拷贝数的增加。注射重组DNM1、DNM2或DNM3蛋白可抑制WSSV在体内的复制。总之，我们的研究表明了宿主miRNAs通过调节宿主JAK/STAT途径在促进WSSV复制中的作用。
MicroRNAs (miRNAs) have important roles in post-transcriptional regulation of gene expression. During viral infection, viruses utilize hosts to enhance their replication by altering cellular miRNAs. The Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway plays crucial roles in the antiviral responses. In this study, two miRNAs (miR-9041 and miR-9850) from Macrobrachium rosenbergii were found to promote white spot syndrome virus (WSSV) replication. The up-regulation of miR-9041 or miR-9850 suppresses STAT expression in the gills of M. rosenbergii, which subsequently down-regulates the expression of its downstream dynamin (Dnm) genes: Dnm1, Dnm2, and Dnm3. Knockdown of miR-9041 and miR-9850 restricts WSSV replication by up-regulating STAT and Dnm gene expression. The silencing of STAT, Dnm1, Dnm2, or Dnm3 led to an increase of the number of WSSV copies in shrimp. The injection of recombinant Dnm1, Dnm2, or Dnm3 proteins could inhibit WSSV replication in vivo. Overall, our research indicates the roles of host miRNAs in the enhancement of WSSV replication by regulating the host JAK/STAT pathway.