The origin, kinetics, and characteristics of the Kupffer cells in the normal steady state.
The origin, kinetics, and characteristics of the Kupffer cells in the normal steady state.
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DOI：
10.1084/jem.148.1.1
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发表时间：
1978-07-01
期刊：
影响因子：
--
通讯作者：
van Furth R
中科院分区：
文献类型：
--
作者：
Crofton RW;Diesselhoff-den Dulk MM;van Furth R
使用链霉蛋白酶和 DNA 酶进行酶消化，从小鼠肝脏中分离库普弗细胞。这些细胞的特征与腹膜巨噬细胞相似，只是在初始悬浮液中，具有Fc受体的Kupffer细胞百分比较低，缺乏C受体，并且由于链霉蛋白酶对细胞膜的影响，对opsenized细菌的摄取非常差。体外培养 24 小时后，所有这些特征都会恢复。 Kupffer 细胞的体外和 1 小时脉冲 [(3)H]胸苷标记较低（分别为 0.8% 和 1%），表明这些细胞本质上不分裂。研究还表明，一小部分体外标记的库普弗细胞最近来自循环系统。静脉注射酵母聚糖后，库普弗细胞的体外标记指数增加了16倍，但事实证明，这些分裂细胞是最近从骨髓中招募的不成熟的单核吞噬细胞。库普弗细胞的标记在注射一次或四次[(3)H]胸苷后分别在48小时时达到10.4%的峰值或在60小时时达到24.1%的峰值，表明这些细胞源自标记的单核细胞。这一结论的进一步证据是通过氢化可的松治疗期间标记的库普弗细胞没有增加而获得的，氢化可的松会导致单核细胞减少，在此期间没有循环单核细胞可迁移到组织。对用后肢屏蔽进行 X 射线照射的动物进行的标记研究显示，库普弗细胞标记指数为正常值的 5-10%，这证实了它们的骨髓来源。对骨髓中标记单核细胞的产生及其通过循环的转运的定量研究表明，在正常稳定状态下，离开循环的单核细胞中至少有 56.4% 变成库普弗细胞。考虑到库普弗细胞在动力学上是同质的，这给出了库普弗细胞总群体的平均周转时间为 21 天。
Enzymatic digestion with pronase and DNAase was used to isolate Kupffer cells from mouse liver. The characteristics of these cells were found to be similar to those of peritoneal macrophages, except that in the initial suspension the percentage of Kupffer cells with Fc receptors was low, C receptors were absent and the ingestion of opsenized bacteria was very poor, because of the effect of pronase on the cell membrane. After 24 h incubation in vitro all these characteristics return. The in vitro and 1 h-pulse [(3)H]thymidine labeling of the Kupffer cells is low (0.8 and 1 percent, respectively) indicating that in essence these cells do not divide. It was also shown that the small percentage of in vitro labeled Kupffer cells was recently derived from the circulation. After an intravenous injection of zymosan the in vitro labeling index of the Kupffer cells increased 16-fold, but it was proven that these dividing cells were immature mononuclear phagocytes very recently recruited from the bone marrow. The labeling of Kupffer cells aider one or four injections of [(3)H]thymidine reached a peak of 10.4 percent at 48 h or 24.1 percent at 60 h, respectively, indicating that these cells are derived from labeled monocytes. Further evidence for this conclusion was obtained by the absence of an increase of labeled Kupffer cells during treatment with hydrocortisone, which causes a monocytopenia during which no circulating monocytes are available to migrate to the tissues. Labeling studies in animals X-irradiated with hind-limb shielding gave a Kupffer cell labeling index of 5-10 percent of the normal values, which confirms their bone marrow origin. A quantitative study on the production of labeled monocytes in the bone marrow and their transit through the circulation showed that in the normal steady state at least 56.4 percent of the monocytes leaving the circulation become Kupffer cells. Considering the Kupffer cells as kinetically homogeneous this gives a mean turnover time of the total population of Kupffer cells of 21 days.