THE EFFECT OF EXTRACTS OF CERTAIN ORGANS FROM NORMAL AND IMMUNIZED ANIMALS ON THE INFECTING POWER OF VACCINE VIRUS.
THE EFFECT OF EXTRACTS OF CERTAIN ORGANS FROM NORMAL AND IMMUNIZED ANIMALS ON THE INFECTING POWER OF VACCINE VIRUS.
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DOI：
10.1084/jem.50.3.327
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发表时间：
1929-08-31
期刊：
影响因子：
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通讯作者：
Duran-Reynals F
中科院分区：
文献类型：
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作者：
Duran-Reynals F
免疫兔子的大脑和睾丸组织与Levaditi或Noguchi株疫苗病毒接触后，将修复或灭活病毒。易感动物的正常睾丸提取物极大地增强了疫苗病毒神经株和真皮株的传染性。当注射到皮肤中时，野口病毒不会受到睾丸提取物的影响，但当注射到皮肤或睾丸中时，肾脏提取物对病毒有一定的增强作用。组织提取物似乎对宿主的细胞起作用，而不是对病毒起作用。静脉注射的病毒最容易定位在以前注射了睾丸提取液的皮肤区域，这一事实表明了这一点。此外，如果在注射睾丸提取液后的3天内将病毒注射到该区域，则会导致强化的损害。组织提取物的增强物质几乎不受高稀释的影响，通过Berkefeld V蜡烛，并与弱酸沉淀的蛋白质一起携带。病变增强的兔子表现出一般症状，约25%的兔子死于全身性牛痘。肾脏，可能还有皮肤、大脑和肝脏提取物都有增强作用，但程度比睾丸小得多。另一方面，脾、血液以及可能的淋巴结和骨髓不仅没有产生增强作用，反而抑制甚至完全抑制了疫苗的皮肤感染。
Brain and testicle tissue from immune rabbits brought in contact with the Levaditi or Noguchi strains of vaccine virus will fix or inactivate the virus. Extracts of normal testicles from susceptible animals enhance to an extraordinary degree the infectivity of both the neuro- and dermal strains of vaccine virus. The Noguchi virus is not affected by testicle extracts when injected into the skin, but kidney extract has a definite enhancing power on the strain when injected into either skin or testicle. The effect of tissue extracts seems to be on the cells of the host rather than on the virus. This is indicated by the fact that virus injected intravenously localizes most readily in an area of skin previously injected with testicle extract. Furthermore an enhanced lesion results if the virus is injected into an area as long as 3 days after the area has been injected with testicle extract. The augmenting substance of the tissue extracts is little affected by high dilutions, passes through a Berkefeld V candle and is carried down with the proteins precipitated by weak acids. Rabbits with enhanced lesions show general symptoms and about 25 per cent die with generalized vaccinia. Kidney, and probably skin, brain and liver extracts possess enhancing properties, but to a much less degree than the testicle. On the other hand, spleen, blood and probably lymph nodes and bone marrow not only fail to produce enhancement, but actually restrain or even suppress entirely the vaccinal skin infection.