Interplay of phytohormones facilitate sorghum tolerance to aphids
Interplay of phytohormones facilitate sorghum tolerance to aphids
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DOI：
10.1007/s11103-020-01083-y
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发表时间：
2020-10-15
影响因子：
5.1
通讯作者：
Louis, Joe
中科院分区：
文献类型：
--
作者：
Grover, Sajjan;Agpawa, Earl;Louis, Joe
关键信息植物激素之间的相互作用对于提供高粱植物对蚜虫的耐受性至关重要。植物与食草昆虫的遭遇触发了防御信号网络，微调植物对害虫的抗性。虽然已经确定植物激素有助于抗异源性和抗虫性介导的对害虫的抗性，但它们在调节植物耐受性（最持久和最有希望的宿主植物抗性类别）中的作用在很大程度上是未知的。在这里，我们筛选了一组高粱（高粱双色）自交系，以确定和表征高粱对甘蔗蚜虫（SCA;Melanaphis sacchari Zehntner）的耐受性，这是一种相对较新的破坏性高粱害虫。我们的研究结果表明，高粱基因型SC 35，高粱基因型中确定的蚜虫耐受线，显示最小的植物生物量损失和强大的光合机制，尽管支持更高的蚜虫人口。植物激素分析显示，在高粱SCA耐受性SC 35植物中，茉莉酸生物合成途径中的前体12-氧代-植物二烯酸的基础水平显著较高。水杨酸的积累似乎是高粱植株对蚜虫的一种普遍的植物反应，然而，SCA饲喂诱导的水杨酸水平在高粱耐受基因型中没有改变。相反，基础水平的脱落酸和蚜虫喂养诱导的细胞分裂素积累在SCA耐受高粱基因型。我们的研究结果表明，蚜虫耐受高粱基因型严格控制植物激素之间的关系，以及提供重要的见解的潜在机制，有助于植物耐受吸汁蚜虫。
Key message Interactions among phytohormones are essential for providing tolerance of sorghum plants to aphids. Plant's encounter with insect herbivores trigger defense signaling networks that fine-tune plant resistance to insect pests. Although it is well established that phytohormones contribute to antixenotic- and antibiotic-mediated resistance to insect pests, their role in conditioning plant tolerance, the most durable and promising category of host plant resistance, is largely unknown. Here, we screened a panel of sorghum (Sorghum bicolor) inbred lines to identify and characterize sorghum tolerance to sugarcane aphids (SCA;Melanaphis sacchari Zehntner), a relatively new and devastating pest of sorghum in the United States. Our results suggest that the sorghum genotype SC35, the aphid-tolerant line identified among the sorghum genotypes, displayed minimal plant biomass loss and a robust photosynthetic machinery, despite supporting higher aphid population. Phytohormone analysis revealed significantly higher basal levels of 12-oxo-phytodienoic acid, a precursor in the jasmonic acid biosynthesis pathway, in the sorghum SCA-tolerant SC35 plants. Salicylic acid accumulation appeared as a generalized plant response to aphids in sorghum plants, however, SCA feeding-induced salicylic acid levels were unaltered in the sorghum tolerant genotype. Conversely, basal levels of abscisic acid and aphid feeding-induced cytokinins were accumulated in the SCA-tolerant sorghum genotype. Our findings imply that the aphid-tolerant sorghum genotype tightly controls the relationship among phytohormones, as well as provide significant insights into the underlying mechanisms that contribute to plant tolerance to sap-sucking aphids.