The Combined Effect of Sleep Deprivation and Western Diet on Spatial Learning and Memory: Role of BDNF and Oxidative Stress
The Combined Effect of Sleep Deprivation and Western Diet on Spatial Learning and Memory: Role of BDNF and Oxidative Stress
复制标题
DOI：
10.1007/s12031-012-9881-7
复制
发表时间：
2013-05-01
影响因子：
3.1
通讯作者：
Abu Rashid, Baraa E.
中科院分区：
文献类型：
--
作者：
Alzoubi, Karem H.;Khabour, Omar F.;Abu Rashid, Baraa E.
无论是睡眠剥夺还是西方饮食都可以通过诱导氧化应激来损害学习和记忆，从而导致神经元损伤和神经传递干扰。在这项研究中，我们研究了睡眠剥夺和西方饮食的联合作用对大脑皮层依赖性空间学习和记忆的影响。此外，研究了睡眠剥夺和西方饮食诱导的认知障碍的可能分子靶点。使用改良的多平台模型在大鼠中诱导睡眠剥夺，同时给予西方饮食6周。随后，使用径向臂水迷宫测试空间学习和记忆。在分子水平，BDNF蛋白和抗氧化标记物，包括超氧化物歧化酶（SOD），过氧化氢酶，谷胱甘肽过氧化物酶（GPx），谷胱甘肽（GSH），氧化型谷胱甘肽（GSSG），GSH/GSSG，和硫代巴比妥酸反应物质（TBARS）进行了评估。研究结果显示，睡眠剥夺、西方饮食或两者结合都会损害短期和长期记忆（P < 0.05）。联合用药组24 h长时记忆损害程度明显高于各因素单独用药组（P < 0.05）。此外，联合治疗降低了海马脑源性神经营养因子的水平，而单独使用每种因素时均未检测到这种降低。联合用药可降低海马SOD、CAT、GPx活性，降低GSH/GSSG比值（P < 0.05），升高TBARS水平（P < 0.05）。总之，睡眠剥夺和西方饮食的结合降低了BDNF水平，增加了海马体中的氧化应激，从而诱导了比单独使用每种因素产生的损伤更大的记忆损伤。
Either sleep deprivation or Western diet can impair learning and memory via induction of oxidative stress, which results in neuronal damage and interference with the neurotransmission. In this study, we examined the combined effect of sleep deprivation and Western diet on hippocampus-dependent spatial learning and memory. In addition, possible molecular targets for sleep deprivation and Western diet-induced cognitive impairments were investigated. Sleep deprivation was induced in rats using the modified multiple platform model simultaneous with the administration of Western diet for 6 weeks. Thereafter, spatial learning and memory were tested using radial arm water maze. At the molecular level, BDNF protein and antioxidant markers including superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), glutathione (GSH), oxidized glutathione (GSSG), GSH/GSSG, and thiobarbituric acid reactive substances (TBARS) were assessed. The results of this study revealed that sleep deprivation, Western diet, or a combination of both impair short- and long-term memory (P < 0.05). The magnitude of the impairment induced by the combined treatment at the 24-h long-term memory was higher than that caused by each factor alone (P < 0.05). In addition, the combined treatment reduced the levels of hippocampal BDNF, a reduction that was not detected with each factor alone. Moreover, the combined treatment reduced the hippocampal activities of SOD, catalase, GPx, ratio of GSH/GSSG, and elevated TBARS level (P < 0.05). In conclusion, the combination of sleep deprivation and Western diet decreases BDNF levels and increases oxidative stress in the hippocampus, thus inducing memory impairment that is greater than the impairment produced by each factor alone.