T1ρ imaging as a non-invasive assessment of collagen remodelling and organization in human skeletal muscle after ligamentous injury.
T1ρ imaging as a non-invasive assessment of collagen remodelling and organization in human skeletal muscle after ligamentous injury.
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DOI：
10.1113/jp281964
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发表时间：
2021-12
期刊：
影响因子：
--
通讯作者：
Fry CS
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文献类型：
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作者：
Noehren B;Hardy PA;Andersen A;Brightwell CR;Fry JL;Vandsburger MH;Thompson KL;Fry CS
骨骼肌细胞外基质（ECM）的失调和纤维化是损伤的结果。目前的ECM评估需要肌肉活检来评估肌肉ECM的改变，这在人类中通常是不实用的。本研究的目的是评估量化T1ρ松弛时间的磁共振成像序列预测ECM胶原组成和组织的潜力。对27例前交叉韧带（ACL）撕裂患者进行T1ρ成像并对受累和未受累股外侧肌进行肌肉活检。通过拟合一系列T1ρ加权图像的单指数衰减曲线来量化T1ρ次数。除羟脯氨酸外，采用免疫组织化学和组织化学方法获得胶原含量和组织等ECM指标。采用多元线性回归模型选择来评估T1ρ倍与ECM组成之间的关系。此外，比较acl缺陷和健康肢体，以确定T1ρ的敏感性，以检测损伤后肌肉ECM的早期适应性。我们发现，T1ρ松弛时间与acl缺失肢体的胶原展开（t=4.093, p=0.0007）和健康肢体的胶原1丰度（t=2.75, p=0.014）密切相关。此外，我们发现损伤肢体的T1ρ松弛时间明显更长，这与acl缺失肢体胶原含量和重塑的几个指标的显著差异相吻合。这些结果支持使用T1ρ以非侵入性方式评估骨骼肌中的ECM组成。
Dysregulation and fibrosis of the extracellular matrix (ECM) in skeletal muscle is a consequence of injury. Current ECM assessment necessitates muscle biopsies to evaluate alterations to the muscle ECM, which is often not practical in humans. The goal of this study was to evaluate the potential of a magnetic resonance imaging sequence that quantifies T1ρ relaxation time to predict ECM collagen composition and organization. T1ρ imaging was performed and muscle biopsies obtained from the involved and non-involved vastus lateralis muscle on 27 subjects who had an anterior cruciate ligament (ACL) tear. T1ρ times were quantified via mono exponential decay curve fitted to a series of T1ρ-weighted images. Several ECM indices, including collagen content and organization, were obtained using immunohistochemistry and histochemistry in addition to hydroxyproline. Model selection with multiple linear regression was used to evaluate the relationships between T1ρ times and ECM composition. Additionally, the ACL-deficient and healthy limb were compared to determine sensitivity of T1ρ to detect early adaptations in the muscle ECM following injury. We show that T1ρ relaxation time was strongly associated with collagen unfolding (t=4.093, p=0.0007) in the ACL-deficient limb, and collagen 1 abundance in the healthy limb (t=2.75, p=0.014). In addition, we show that T1ρ relaxation time is significantly longer in the injured limb, coinciding with significant differences in several indices of collagen content and remodeling in the ACL-deficient limb. These results support the use of T1ρ to evaluate ECM composition in skeletal muscle in a non-invasive manner.