In vitro and in vivo imaging of peroxynitrite by a ratiometric boronatebased fluorescent probe
In vitro and in vivo imaging of peroxynitrite by a ratiometric boronatebased fluorescent probe
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DOI：
10.1016/j.bios.2017.01.027
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发表时间：
2017-05-15
影响因子：
12.6
通讯作者：
Wang, Yun-Ming
中科院分区：
文献类型：
--
作者：
Palanisamy, Sathyadevi;Wu, Pei-Yu;Wang, Yun-Ming
过氧亚硝酸盐(ONOO-)是参与多种生理病理过程的重要物种。在开发新型荧光探针检测过氧亚硝酸盐方面取得了进展，具有相对较高的灵敏度和特异度。在这里，我们报道了一种新的基于硼酸盐的荧光探针4-MB的合成、表征和生物学应用。研究表明，由于ONOO引发的氧化反应，4-MB对过氧亚硝酸根表现出比率和量热双重响应。提出了氧化反应的可能机理，并用不同的光谱方法对反应产物进行了分离和表征。我们已经彻底证明了4-MB用于细胞内过氧亚硝酸根成像的实用性。此外，我们还发现，4-MB可用于显示RAW264.7巨噬细胞、EAhy926细胞、斑马鱼和高脂饮食诱导肥胖小鼠模型的活组织中外源性和内源性过氧亚硝酸根。
Peroxynitrite (ONOO-) is an important species involved in many physiopathological processes. Progresses have been made in developing novel fluorescent probes to detect peroxynitrite with relatively high sensitivity and specificity. Herein, we report the synthesis, characterization and biological applications of a new boronate-based fluorescent probe, 4-MB. The studies showed that 4-MB exhibits a dual ratiometric and calorimetric response toward peroxynitrite due to ONOO--triggered oxidative reaction. A possible mechanism of the oxidation reaction was proposed and the reaction product was isolated and characterized using different spectroscopic methods. We have thoroughly demonstrated the utility of 4-MB for intracellular peroxynitrite imaging. Further, we showed that 4-MB can be potentially employed to visualize exogenous and endogenous peroxynitrite in RAW264.7 macrophages, EAhy926 cells, zebrafish and in live tissues from a high-fat diet-induced obese mouse model.