Changes in hepatic gene expression associated with the hypocholesterolaemic activity of royal jelly
Changes in hepatic gene expression associated with the hypocholesterolaemic activity of royal jelly
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DOI：
10.1211/jpp.58.12.0017
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发表时间：
2006-12-01
影响因子：
3.3
通讯作者：
Sakaki, Toshiyuki
中科院分区：
文献类型：
--
作者：
Kamakura, Masaki;Moriyama, Tatsuya;Sakaki, Toshiyuki
皇家果冻（RJ）在实验动物中具有多种药理作用，包括降血脂、降胆固醇和抗动脉粥样硬化作用，但其分子机制尚不清楚。在这里，我们调查的脂质代谢相关基因在RJ处理的小鼠肝脏中的表达的变化，通过DNA微阵列技术，以获得线索的RJ的降胆固醇作用的机制。我们比较了三组小鼠的肝脏基因表达谱，这些小鼠分别喂食含有5% RJ的饮食、在40摄氏度下储存7天（40- 7天RJ）的含有5% RJ的饮食或提供与其他饮食相同总能量的对照饮食。RJ降低了胆固醇生物合成关键酶鲨烯环氧酶（SQLE）和可能是SQLE转录因子的固醇调节元件结合蛋白（SREB）-1的基因表达。它增加了低密度脂蛋白受体（LDLR）的基因表达，该受体参与肝脏中胆固醇的掺入。因此，RJ的降胆固醇作用似乎与小鼠SQLE降低和LDLR增加有关。
Royal jelly (RJ) has various pharmacological actions, including hypolipidaemic, hypocholesterolaemic and anti-atherosclerotic effects, in experimental animals but the molecular mechanisms involved remain unclear. Here, we investigated changes in the expression of lipid metabolism-associated genes in the liver of RJ-treated mice by means of a DNA microarray technique to obtain clues to the mechanism of the hypocholesterolaemic action of RJ. We compared the hepatic gene expression profiles in three groups of mice fed a diet containing 5% RJ, a diet containing 5% RJ stored at 40 degrees C for 7 days (40-7d RJ) or a control diet which provided the same total energy as the other diets. RJ decreased gene expression of squalene epoxidase (SQLE), which is a key enzyme in cholesterol biosynthesis, and sterol regulatory element-binding protein (SREB)-1, which may be a transcriptional factor of SQLE. It increased gene expression of low-density lipoprotein receptor (LDLR), which is involved in cholesterol incorporation in liver. Thus, the hypocholesterolaemic action of RJ appears to be associated with a decrease of SQLE and an increase of LDLR in mice.