Possible tumor suppressive role of the miR-144/451 cluster in esophageal carcinoma as determined by principal component regression analysis
Possible tumor suppressive role of the miR-144/451 cluster in esophageal carcinoma as determined by principal component regression analysis
复制标题
主成分回归分析确定 miR-144/451 簇在食管癌中可能的肿瘤抑制作用
DOI：
10.3892/mmr.2016.5691
复制
发表时间：
2016-10-01
影响因子：
3.4
通讯作者：
Pu, Yuepu
中科院分区：
文献类型：
--
作者：
Gao, Zhikui;Liu, Ran;Pu, Yuepu
microRNA（miRNA）簇在不同类型的生物体中普遍表达，并且越来越多的研究表明，在某些癌症类型的发展过程中，与单个miRNA相比，miRNA簇的功能更有效。作为诊断或治疗生物标志物，miRNA簇可能比单个miRNA具有更高的稳定性和可靠性。在本研究中，使用茎环逆转录-定量聚合酶链反应检测了102例病理诊断为食管癌的患者中miR-144/451簇内成熟miRNA的表达水平。使用生物信息学工具来鉴定miR-144/451簇的可能的miRNA介导的网络。hsa-miR-451 a、hsa-miR-144- 3 p和hsa-miR-144- 5 p在肿瘤组织中的表达水平显著低于癌旁组织（P
MicroRNA (miRNA) clusters are expressed universally across different types of organisms, and an accumulating number of studies have demonstrated that miRNA clusters function more efficiently compared with single miRNAs during the development of certain cancer types. miRNA clusters may have increased stability and reliability over individual miRNAs as diagnostic or therapeutic biomarkers. In the present study, the expression levels of mature miRNAs within the miR-144/451 cluster were examined using stem-loop reverse transcription-quantitative polymerase chain reaction in 102 patients pathologically diagnosed with esophageal carcinoma. Bioinformatics tools were used to identify a possible miRNA-mediated network of the miR-144/451 cluster. The expression levels of hsa-miR-451a, hsa-miR-144-3p and hsa-miR-144-5p in tumor tissues were significantly lower compared with those in adjacent non-tumor tissues (P