Differences in anti-inflammatory effects between two specifications of Scutellariae Radix in LPS-induced macrophages in vitro
Differences in anti-inflammatory effects between two specifications of Scutellariae Radix in LPS-induced macrophages in vitro
复制标题
两种规格黄芩对LPS诱导巨噬细胞体外抗炎作用的差异
DOI：
10.1016/s1875-5364(17)30077-8
复制
发表时间：
2017-07-01
影响因子：
4.6
通讯作者：
Cai Shao-Qing
中科院分区：
文献类型：
--
作者：
Chen Qian-Yu;Wang Chao-Qun;Cai Shao-Qing
黄芩是黄芩的根，具有解热、抗炎作用。根据中医理论，SR分为“苦芩”（KQ）和“紫芩”（ZQ）两种规格，用于不同的症状（上焦热或三焦下）。然而，尚未确定这两种质量标准的有效性差异。在本研究中，我们的目的是表征KQ和ZQ之间的抗炎活性的差异，并探讨它们的差异是如何表现在脂多糖（LPS）诱导的巨噬细胞。结果表明，在小鼠腹腔巨噬细胞系RAW 264. 7细胞中，KQ和ZQ均能抑制一氧化氮（NO）、诱导型一氧化氮合酶（iNOS）和核因子-κ B（NF-κ B）的释放，并呈剂量依赖性，但无显著差异。在NR 8383细胞（大鼠肺泡巨噬细胞系）中，KQ和ZQ对NO、iNOS和NF-κ B的作用与RAW264.7细胞相似，但KQ对NO和iNOS的抑制率高于ZQ。结果表明，止咳、镇咳两药治疗肺部炎症的疗效存在差异。本研究结果为中医药文献中记载的KQ和ZQ在临床上的不同用途提供了实验依据。
Scutellariae Radix (SR), the root of Scutellaria baicalensis Georgi, is used as an antipyretic drug and has been demonstrated to have anti-inflammatory activity. SR is divided into two specifications, "Ku Qin" (KQ) and "Zi Qin" (ZQ), for use against different symptoms (upper energizer heat or lower portion of the triple energizer), according to the theory of traditional Chinese medicine (TCM). However, differences in the efficacies of these two specifications have not been determined. In the present study, we aimed to characterize the differences in the anti-inflammatory activities between KQ and ZQ and to explore how their differences are manifested in lipopolysaccharide (LPS)-induced macrophages. Our results showed that, in RAW264.7 cells (a mouse macrophage cell line derived from ascites), KQ and ZQ displayed anti-inflammatory effects by inhibiting the release of nitric oxide (NO), inducible NOS (iNOS), and nuclear factor-kappa B (NF-kappa B) in a dose-dependent manner without distinction. In NR8383 cells (a rat alveolar macrophage cell line), KQ and ZQ displayed similar effects on NO, iNOS, and NF-kappa B as seen in RAW264.7 cells, but KQ showed a higher inhibition rate for NO and iNOS than that shown by ZQ at the same concentration. These results indicated that there were differences in efficacy between KQ and ZQ in treating lung inflammation. Our findings provided an experimental evidence supporting the different uses of KQ and ZQ in clinic, as noted in ancient herbal records.