CALCIUM ENHANCES THE HEMOLYTIC ACTION OF BILE-SALTS
CALCIUM ENHANCES THE HEMOLYTIC ACTION OF BILE-SALTS
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DOI：
10.1016/0005-2736(86)90081-7
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发表时间：
1986-03-13
期刊：
影响因子：
--
通讯作者：
RAFTER, J
中科院分区：
文献类型：
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作者：
CHILD, P;RAFTER, J
添加 5-10 mM 氯化钙可显着增强含有等渗盐水的缓冲液中胆汁盐对人红细胞的裂解作用。所有测试的胆汁酸均显示出这种效应，在 10 mM 氯化钙存在下，在 0.75 mM 的去氧胆酸盐、1 mM 的鹅去氧胆酸盐、2.5 mM 的熊去氧胆酸盐和 5.5 mM 的胆酸盐下，裂解发生显着增加。这种效应似乎是针对钙的。氯化锶和氯化镁没有产生刺激作用。在 1 mM 脱氧胆酸盐和 1-10 mM 氯化钙存在下，红细胞裂解的增加与细胞对胆汁盐的吸收增加无关（用[14C]脱氧胆酸盐测量）。使用先前用[3H]胆固醇标记的红细胞，没有证据表明与单独的脱氧胆酸盐相比，在钙和脱氧胆酸盐存在的情况下增强了该膜成分的去除。在 1 mM 脱氧胆酸盐存在下，细胞对钙作用的敏感性随着其在 4°C 下储存时间的延长而增加。 C. 37℃孵育后灵敏度恢复至新鲜细胞的灵敏度。 C 用 30 mM 腺苷加 25 mM 葡萄糖，但这种处理并没有进一步减少裂解。在 10 mM 氯化钙和 1 mM 脱氧胆酸盐存在下的裂解通过增加 KCl 浓度（以 NaCl 为代价）而被部分阻断。使用包含 100 mM KCl/50 mM NaCl 的缓冲液时效果最大。将钙螯合剂 quin2 掺入细胞后，裂解现象更加显着减少。与对照组相比，在含有 quin2 的细胞中，在钙存在的情况下由 1 mM 脱氧胆酸盐诱导的裂解减少了 80%。数据表明，胆汁酸可以促进钙流入红细胞，由于细胞内钾的流出和/或从培养介质中吸收钠而导致裂解。数据进一步表明，细胞效应可能在较低的胆汁酸浓度下发生，该浓度被认为是洗涤剂损坏所需的。
The lysis of human erythrocytes by bile salts in buffer containing isotonic saline was dramatically enhanced by the addition of 5-10 mM calcium chloride. All bile acids tested showed this effect, with a marked increase in lysis occurring at 0.75 mM for dexoycholate, 1 mM for chenodeoxycholate, 2.5 mM for ursodeoxycholate and 5.5 mM with cholate in the presence of 10 mM calcium chloride. The effect appeared to be specific for calcium; strontium chloride and magnesium chloride gave no stimulatory effect. The increased lysis of the erythrocytes in the presence of 1 mM deoxycholate and 1-10 mM calcium chloride was not associated with increased uptake of the bile salt by the cells (measured with [14C]deoxycholate). Using erythrocytes previously labelled with [3H]cholesterol, there was no evidence of an enhanced removal of that membrane component in the presence of calcium and deoxycholate, compared to deoxycholate alone. The sensitivity of the cells to the effect of calcium in the presence of 1 mM deoxycholate increased with the length of time of their storage at 4.degree. C. The sensitivity returned to that of fresh cells after incubation at 37.degree. C with 30 mM adenosine plus 25 mM glucose, but this treatment did not further diminish the lysis. Lysis in the presence of 10 mM calcium chloride and 1 mM deoxycholate was partially blocked by increasing the KCl concentration at the expense of NaCl. The maximum effect occurred with a buffer comprising 100 mM KCl/50 mM NaCl. A more dramatic reduction in the lysis followed the incorporation of the calcium chelator, quin2, into the cells. The lysis induced by 1 mM deoxycholate in the presence of calcium was reduced by 80% in quin2-loaded cells compared to controls. The data suggest that bile acids can promote the influx of calcium into erythrocytes, leading to lysis as a result of the efflux of intracellular potassium and/or the uptake of sodium from the incubation medium. The data further suggest that cellular effects may occur at lower bile acid concentrations that that thought to be required for detergent damage.