Identification of the chlE gene encoding oxygen-independent Mg-protoporphyrin IX mono methyl ester cyclase in cyanobacteria
Identification of the chlE gene encoding oxygen-independent Mg-protoporphyrin IX mono methyl ester cyclase in cyanobacteria
复制标题
蓝藻中编码不依赖氧的镁原卟啉 IX 单甲酯环化酶的 ChlE 基因的鉴定
DOI：
10.1016/j.bbrc.2015.06.124
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发表时间：
2015
影响因子：
3.1
通讯作者：
Y.
中科院分区：
文献类型：
--
作者：
Yamanashi;K.;Minamizaki;K. and Fujita;Y.
叶绿素(Chl)A的第五环(E环)是由镁原卟啉IX单甲酯(MPE)环化酶产生的。有两种进化上不相关的MPE循环酶：氧非依赖性(BChE)和氧依赖(Chla/ACSF)MPE循环酶。虽然Chla是聚球藻PCC6803中唯一的MPE循环酶，但目前还不清楚蓝藻中是否存在BChE。BLAST搜索表明，只有极少数蓝藻拥有bchE。在这里，我们报道了来自氰硫杆菌PCC7425和PCC7822的两个候选基因恢复了abchE缺失的胶囊红杆菌突变体的光合作用生长和细菌叶绿素的产生。我们将这些蓝藻称为“CHLE”。
The fifth ring (E-ring) of chlorophyll (Chl)ais produced by Mg-protoporphyrin IX monomethyl ester (MPE) cyclase. There are two evolutionarily unrelated MPE cyclases: oxygen-independent (BchE) and oxygen-dependent (ChlA/AcsF) MPE cyclases. Although ChlA is the sole MPE cyclase inSynechocystisPCC 6803, it is yet unclear whether BchE exists in cyanobacteria. A BLAST search suggests that only few cyanobacteria possessbchE. Here, we report that twobchEcandidate genes fromCyanothecestrains PCC 7425 and PCC 7822 restore the photosynthetic growth and bacteriochlorophyll production in abchE-lacking mutant ofRhodobacter capsulatus. We termed these cyanobacterialbchEorthologs “chlE.”