The arsenite oxidase genes (aroAB) in novel chemoautotrophic arsenite oxidizers
The arsenite oxidase genes (aroAB) in novel chemoautotrophic arsenite oxidizers
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DOI：
10.1016/j.bbrc.2007.01.004
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发表时间：
2007-03-16
影响因子：
3.1
通讯作者：
Young, L. Y.
中科院分区：
文献类型：
--
作者：
Rhine, E. D.;Ni Chadhain, S. M.;Young, L. Y.
从环境中分离到6株新的细菌菌株，它们可以在有氧或厌氧条件下将亚砷酸盐[As（III）]氧化为移动的较少的砷酸盐[As（V）]，并与CO2固定相结合。针对亚砷酸盐氧化酶大亚基的保守结构域设计引物，对分离菌株的亚砷酸盐氧化酶基因进行PCR鉴定。本文报道的亚砷酸盐氧化酶的氨基酸序列与先前报道的唯一自养亚砷酸盐氧化剂NT-26菌株的氨基酸序列有72-74%的相同性。事实上，自养型亚砷酸盐氧化酶基因形成了一个独特的系统发育组，与先前描述的异养型亚砷酸盐氧化酶基因分开，除了异养型根癌土壤杆菌。亚砷酸盐氧化酶引物在这里描述代表了一个强大的文化独立的工具，以评估环境细菌中的亚砷酸盐氧化酶基因的多样性。(c)2007年爱思唯尔公司All rights reserved.
Six novel bacterial strains were isolated from the environment which can oxidize arsenite [As(III)] to the less mobile form arsenate [As(V)] coupled to CO2 fixation under either aerobic or denitrifying conditions. PCR primers were designed to the conserved molybdopterin domain of the large subunit of arsenite oxidase in order to identify the arsenite oxidase genes from these isolates. The amino acid sequences for the arsenite oxidases reported here were 72-74% identical to that of strain NT-26, the only previously reported autotrophic arsenite oxidizer. Indeed the autotrophic arsenite oxidase genes form a distinct phylogenetic group, separated from previously described heterotrophic arsenite oxidase genes, with the exception of the heterotroph Agrobacterium tumefaciens. The arsenite oxidase primers described here represent a powerful culture-independent tool to assess the diversity of arsenite oxidase genes in environmental bacteria. (c) 2007 Elsevier Inc. All rights reserved.