Characterisation of cytolethal distending toxin (CDT) mutants of Campylobacter jejuni
Characterisation of cytolethal distending toxin (CDT) mutants of Campylobacter jejuni
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DOI：
10.1099/0022-1317-49-5-473
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发表时间：
2000-05-01
影响因子：
3
通讯作者：
Leach, SA
中科院分区：
文献类型：
--
作者：
Purdy, D;Buswell, CM;Leach, SA
为了评估细胞致死膨胀毒素（CDT）对空肠弯曲杆菌毒力和致病性的贡献，通过插入cdtB毒素亚基突变使C.空肠81-176和C.空肠NCTC 11168 CDTs失活，在HeLa细胞毒实验中发现，等基因C、空肠81-176 cdtB（-）菌株的无细胞声索明显减弱，但仍保留一定的毒力。当给成年严重联合免疫缺陷（SCID）小鼠口服时，空肠C. cdtB突变株的肠道定植能力不受影响，但对血液、脾脏和肝脏组织的侵袭能力受损。这些数据表明CDT可能是该物种产生的主要毒素，并且一些空肠梭菌菌株可能产生与CDT不同的其他产毒因子。
In order to assess the contribution of cytolethal distending toxin (CDT) to the toxigenicity and pathogenicity of Campylobacter jejuni, the C. jejuni 81-176 and C. jejuni NCTC 11168 CDTs were inactivated by insertional mutation of the cdtB toxin subunit, Cell-free sonicates from isogenic C, jejuni 81-176 cdtB(-) strains were found to be greatly attenuated in HeLa cytotoxicity assays, whilst still retaining some toxigenicity, Sonicates from a C, jejuni NCTC 11168 cdtB(-) strain produced no detectable cytotoxicity When orally administered to adult severe combined immunodeficient (SCID) mice, C. jejuni cdtB mutant strains were unaffected in enteric colonisation abilities but demonstrated impaired invasiveness into blood, spleen and liver tissues. These data suggest that CDT may be the principal toxin produced by this species and that some C. jejuni strains may generate additional toxigenic factor(s) distinct from CDT.