Stable carbon isotope ratios for organic acids in commercial honey samples
Stable carbon isotope ratios for organic acids in commercial honey samples
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DOI：
10.1016/j.foodchem.2019.03.053
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发表时间：
2019-08-15
期刊：
影响因子：
8.8
通讯作者：
Suto, Nana
中科院分区：
文献类型：
--
作者：
Kawashima, Hiroto;Suto, Momoka;Suto, Nana
用LC/IRMS测定了116个市售蜂蜜样品中葡萄糖、果糖、二糖、三糖和有机酸的稳定碳同位素比值（Δ C-13）。根据EA/IRMS和LC/IRMS真实性标准，39个样品被判定为掺假。本文首次报道了纯蜂蜜中有机酸的δ C-13值，范围为千分之-33.6至-26.5。平均Delta Delta C-13（葡萄糖-有机酸）值为+3.7 +/- 0.9 ppm。葡萄糖和有机酸δ C-13值呈强相关（R = 0.71，P < 0.001）。葡萄糖酸是蜂蜜中主要的有机酸，据报道是通过蜜蜂葡萄糖氧化酶和某些葡萄糖酸菌属物种分解葡萄糖而产生的。同位素分析证实了这一点。
Stable carbon isotope ratios (delta C-13) for glucose, fructose, disaccharides, trisaccharides, and organic acids in 116 commercial honey samples were measured by LC/IRMS. On the basis of EA/IRMS and LC/IRMS authenticity criteria, 39 of the samples were judged to have been adulterated. The delta C-13 values for organic acids from pure honey, reported here for the first time, ranged from -33.6 to - 26.5 parts per thousand. The mean Delta delta C-13 (glucose-organic acids) value was +3.7 +/- 0.9 parts per thousand. Glucose and organic acid delta C-13 values were strongly correlated (R = 0.71, P < 0.001). Gluconic acid, the predominant organic acid in honey, has been reported to be produced via decomposition of glucose by bee glucose-oxidase and certain Gluconobacter spp. This fact was confirmed by isotope analysis.