An ungrouped cuticular protein is essential for normal endocuticle formation in the brown planthopper
An ungrouped cuticular protein is essential for normal endocuticle formation in the brown planthopper
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未分组的角质层蛋白对于褐飞虱正常内表皮的形成至关重要
DOI：
10.1016/j.ibmb.2018.06.001
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发表时间：
2018-09-01
影响因子：
3.8
通讯作者：
Zhang, Chuan-Xi
中科院分区：
文献类型：
--
作者：
Lu, Jia-Bao;Luo, Xu-Mei;Zhang, Chuan-Xi
利用褐飞虱（BPH），褐飞虱组织的转录组分析，我们确定了一个基因，暂命名为NlCP21.92，在珠被高水平表达。利用定量PCR和Western blotting进行时空表达谱分析，证实了其在皮肤中的特异性表达，并在蜕皮过程中呈周期性表达。开放阅读框富含GC，编码疏水多肽。该多肽含有AAPA/V重复基序和其他序列特征类似于几个报告的表皮蛋白，但缺乏R&R共识和其他几丁质结合结构域。双链RNA介导的RNA干扰NlCP21.92导致异常和致命的形态表型，透射电子显微镜显示相应的超微结构缺陷。免疫组织化学染色显示NICP 21.92蛋白主要位于前表皮。我们的研究结果表明，NICP 21.92是一种新的未分组的表皮蛋白质的正常内表皮形成所必需的。
Using transcriptome analysis of tissues of the brown planthopper (BPH), Nilaparvata lugens, we identified a gene tentatively designated NlCP21.92 that was expressed at high levels in the integument. Spatiotemporal expression profiling with quantitative PCR and Western blotting verified its integument-specific expression and showed periodic expression during molting. The open reading frame was GC-rich and encoded a hydrophobic polypeptide. The polypeptide contained AAPA/V repeat motifs and other sequence features similar to several reported cuticular proteins but lacked an R&R consensus and other chitin-binding domains. Double-stranded RNA-mediated RNA interference of the NlCP21.92 resulted in abnormal and lethal morphological phenotypes, and transmission electron microscopy revealed the corresponding ultrastructural defects. Immunohistochemical staining demonstrated that the NlCP21.92 protein was primarily located in the procuticle. Our results suggest that NlCP21.92 is a novel ungrouped cuticular protein essential for normal endocuticle formation.