THE SURFACE-LOCATED YOPN PROTEIN IS INVOLVED IN CALCIUM SIGNAL TRANSDUCTION IN YERSINIA-PSEUDOTUBERCULOSIS
THE SURFACE-LOCATED YOPN PROTEIN IS INVOLVED IN CALCIUM SIGNAL TRANSDUCTION IN YERSINIA-PSEUDOTUBERCULOSIS
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DOI：
10.1111/j.1365-2958.1991.tb00773.x
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发表时间：
1991-04-01
影响因子：
3.6
通讯作者：
WOLFWATZ, H
中科院分区：
文献类型：
--
作者：
FORSBERG, A;VIITANEN, AM;WOLFWATZ, H
低钙反应(LCR)在致病耶尔森氏菌中是非常保守的，当病原菌在缺钙的介质中以37℃的温度生长时可以观察到LCR。这种反应的特征是普遍的代谢减速和特定的毒力诱导--质粒编码的yop基因。YOP的表达调控是通过温度调节的激活子和钙离子调节的负元件在转录水平上进行的。YopN基因被证明编码一种蛋白质(以前也被命名为Yop4b)，当耶尔森氏菌在37摄氏度生长时，该蛋白质位于表面。YopN被发现是低钙反应过程中诱导的操纵子的一部分。YopN基因插入失活导致yop基因转录失活。在tac启动子的控制下，含有yopN基因的杂交载体完全恢复了yopN突变体的野生型表型。因此，定位于表面的YopN以某种方式感知钙浓度，并在钙浓度较高时发送信号关闭yop转录。
The low-calcium response (lcr) is strongly conserved among the pathogenic Yersinia species and is observed when the pathogen is grown at 37-degrees-C in Ca2+-depleted medium. This response is characterized by a general metabolic downshift and by a specific induction of virulence-plasmid-encoded yop genes. Regulation of yop expression is exerted at transcriptional level by a temperature-regulated activator and by Ca2+-regulated negative elements. The yopN gene was shown to encode a protein (formerly also designated Yop4b) which is surface-located when Yersinia is grown at 37-degrees-C. yopN was found to be part of an operon that is induced during the low-calcium response. Insertional inactivation of the yopN gene resulted in derepressed transcription of yop genes. A hybrid plasmid containing the yopN gene under the control of the tac promoter fully restored the wild-type phenotype of the yopN mutant. Thus the surface-located YopN somehow senses the calcium concentration and transmits a signal to shut off yop transcription when the calcium concentration is high.