Genetic stock structure of the swordfish (Xiphias gladius) inferred by PCR-RFLP analysis of the mitochondrial DNA control region
Genetic stock structure of the swordfish (Xiphias gladius) inferred by PCR-RFLP analysis of the mitochondrial DNA control region
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DOI：
10.1007/s002270050022
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发表时间：
1997-02-01
期刊：
影响因子：
2.4
通讯作者：
Takeyama, H
中科院分区：
文献类型：
--
作者：
Chow, S;Okamoto, H;Takeyama, H
对含有旗鱼 (Xiphiasgladius Linnaeus, 1758) 线粒体 DNA 控制区的 PCR 扩增 DNA 片段进行限制性片段长度多态性 (RFLP) 分析。使用四种核酸内切酶（Alu I、Dde I、Hha I 和 Rsa I）对 13 个本地样本（6 个太平洋样本、3 个大西洋样本、地中海样本、2 个印度洋样本和好望角样本）中的总共 456 个个体进行了调查，总共产生了 52 个复合基因型。样本内基因型多样性 (H) 较高，范围为 0.702 至 0.962，合并样本的值为 0.922。揭示了基因型频率和限制模式的显着地理差异。地中海样本与所有其他样本有很大不同。此外，Rsa I 消化显示除地中海样本外的所有样本中都存在高水平的多态性，表明外源剑鱼很少进入该水体。北大西洋和南大西洋样本之间的异质性显着，两者都不同于太平洋样本。相比之下，印度洋样本与南大西洋和太平洋样本没有显着差异。太平洋样本之间的遗传分化较低。结果表明，全球剑鱼种群的遗传结构不仅在海洋盆地之间，而且在海洋盆地内部也存在，这表明尽管不存在地理障碍，但基因流动受到限制。
Restriction fragment length polymorphism (RFLP) analysis was performed on PCR amplified DNA fragments containing the control region of the swordfish (Xiphias gladius Linnaeus, 1758) mitochondrial DNA. A total of 456 individuals comprising 13 local samples (six Pacific, three Atlantic, the Mediterranean Sea, two Indian Ocean and the Cape of Good Hope) were surveyed with four endonucleases (Alu I, Dde I, Hha I and Rsa I), yielding a total of 52 composite genotypes. Within-sample genotypic diversity (H) was high ranging from 0.702 to 0.962 with a value of 0.922 for the pooled sample. Significant geographic variation in the frequencies of genotypes and restriction patterns was revealed. The Mediterranean sample was highly distinct from all other samples. Further, Rsa I digestion revealed high levels of polymorphism in all but the Mediterranean samples, indicating that exogenous swordfishes rarely enter that body of water. Heterogeneity between the North and South Atlantic samples was significant, both of which differed from those of the Pacific. In contrast, the Indian Ocean samples were not significantly different from the samples of South Atlantic and Pacific. Genetic differentiation among the Pacific samples was low. The results indicate that the worldwide swordfish population is genetically structured not only among, but also within ocean basins and suggest that gene flow is restricted despite the absence of geographic barriers.