The reaction of salivary substances with bacteria.
The reaction of salivary substances with bacteria.
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唾液物质与细菌的反应。
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
H. Wedel
中科院分区：
文献类型：
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作者：
T. Ericson;K. Pruitt;H. Wedel
用分光光度法研究了口腔链球菌与唾液的反应。聚集性唾液细菌混合物的吸光度(700 Nm)随时间的变化曲线呈S型曲线。将吸光度的变化与剩余吸光度的比值与反应时间的对数作图，得到直线。在细菌的固定浓度下，选定唾液样本的一系列稀释的这些直线的斜率是反应混合物中唾液体积的抛物线函数。建立了一个聚集反应模型，解释了这些抛物线的形状。该模型的参数可以从实验数据中计算出来，并可用于估计未稀释的唾液中聚集诱导物质的浓度。发展了一种计算游离细胞与聚集体结合的比速率常数的方法。这个速率常数是给定聚集反应的特征，原则上可以用来区分不同分子结构的聚集诱导物质。
Reactions between selected strains of oral streptococci and saliva were studied spectrophotometrically. The curves of absorbance (700 nm) versus those of time were sigmoidal for aggregating saliva-bacteria mixtures. Whtn the logarithm of the ratio of the change in absorbance to the remaining absorbance was plotted against the reaction time, straight lines were obtained. At a fixed concentration of bacteria, the slope of these lines for a series of dilutions of a selected saliva sample was a parabolic function of the volume of saliva in the reaction mixtures. A model for the aggregation reaction was developed which explained the shape of these parabolic curves. The parameters of the model can be calculated from the experimental data and can be used to estimate the concentration of aggregation-inducing substances in undiluted saliva. A method for calculating the specific rate constant for the binding of free cells to aggregates was developed. This rate constant is a characteristic feature of a given aggregation reaction and can, in principle, be used to distinguish between aggregation-inducing substances of different molecular structures.