PRIMARY STRUCTURE AND FUNCTIONAL EXPRESSION OF A GLUTAMINYL CYCLASE
PRIMARY STRUCTURE AND FUNCTIONAL EXPRESSION OF A GLUTAMINYL CYCLASE
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DOI：
10.1073/pnas.88.22.10059
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发表时间：
1991-11-01
影响因子：
11.1
通讯作者：
SPIESS, J
中科院分区：
文献类型：
--
作者：
POHL, T;ZIMMER, M;SPIESS, J
从牛垂体前叶中分离纯化了一种可能参与焦谷氨酰肽如促性腺激素释放激素和促甲状腺激素释放激素生物合成的β-氨基环化酶（QC）。在N-末端序列分析的基础上，从牛垂体前叶文库中克隆了一个2088 bp的cDNA克隆。从该克隆的核苷酸序列，推导出一个330个残基的蛋白质和前面的31个残基的前原肽序列的一级结构。通过用QC cDNA/pCDM 8载体构建体转染COS-7猴细胞，表达QC活性。与各种牛组织的mRNA杂交显示QC主要在脑组织中表达。
A glutaminyl cyclase (QC) that is probably involved in the biosynthesis of pyroglutamyl peptides such as gonadotropin-releasing hormone and thyrotropin-releasing hormone has been purified to homogeneity from bovine anterior pituitary. On the basis of N-terminal sequence analysis, a 2088-base-pair cDNA clone was isolated from a bovine anterior pituitary library. From the nucleotide sequence of this clone, the primary structure of a 330-residue protein and a preceding 31-residue prepropeptide sequence was deduced. By transfection of COS-7 monkey cells with a QC cDNA/pCDM8 vector construct, QC activity was expressed. Hybridization with mRNAs of various bovine tissues revealed expression of QC mainly in brain tissue.