Isolation and Biological Characteristics of Beijing Fatty Chicken Skeletal Muscle Satellite Cells
Isolation and Biological Characteristics of Beijing Fatty Chicken Skeletal Muscle Satellite Cells
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DOI：
10.3109/15419061.2012.743998
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发表时间：
2012-10-01
影响因子：
--
通讯作者：
Guan, Weijun
中科院分区：
文献类型：
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作者：
Bai, Chunyu;Hou, Lingling;Guan, Weijun
骨骼肌卫星细胞是一种多能干细胞群，在骨骼肌的再生过程中起着重要作用。本研究采用0.1%胶原酶1和0.25%胰蛋白酶联合消化法从15日龄北京油鸡胚胎胸肌中分离骨骼肌卫星细胞。检测到MyoD、Pax7和demin等成肌标志物，表明其骨骼肌卫星细胞身份。核型分析表明，这些体外培养的细胞是遗传稳定的。在骨形成因子和成脂因子的作用下，它们分别分化为骨细胞和脂肪细胞。
Skeletal muscle satellite cells, a postulated multipotential stem cell population, play an essential role in the postnatal replenishment of skeletal muscles. In the present research, the skeletal muscle satellite cells were isolated from the pectorals of 15-day-old Beijing Fatty Chicken embryos using combined enzymatic digestion of 0.1% collagenase 1 and 0.25% trypsin. Myogenic markers such as MyoD, Pax7 and demin were detected, indicating their skeletal muscle satellite cell identity. Karyotype analysis showed that these in vitro cultured cells were genetically stable. Being exposed to bone morphogen and adipogenic factors, it was proved that they differentiated into osteocytes and adipocytes correspondingly.