Clinical studies of the blood volume I Clinical application of a method employing the azo dye "Evans Blue" and the spectrophotometer
Clinical studies of the blood volume I Clinical application of a method employing the azo dye "Evans Blue" and the spectrophotometer
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DOI：
10.1172/jci100859
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发表时间：
1937-05-01
影响因子：
15.9
通讯作者：
Evans, WA Jr
中科院分区：
文献类型：
--
作者：
Gibson, JG II;Evans, WA Jr
本文介绍了用埃文斯蓝染料和分光光度计测定血浆和总血容量的方法在临床问题调查中的应用。由于血浆浑浊、血脂、重复测定中的残留染料和样品溶血，早期方法固有的比色误差通过分光光度计的使用被最小化，并描述了一种校正溶血的分光光度计方法。在不同的临床状态下，由于染料混合时间的变化而产生的误差，以及注射的染料可能被淋巴稀释，可以通过计算血浆体积来消除。通过外推染料从血流中消失的斜率得到的值，如所示。通过在染料注入后至少30分钟的时间内采集多个样品。，到时间的尽头。用“直接”法重复单笔债务。在同一个体中，临床意义的变化可以在频繁的间隔中可靠地测量。通过“间接方法”，可以连续跟踪从几分钟开始的时间内音量的变化。到几个小时。讨论了影响间接法精度的若干因素。对连续采血的生理反应，包括循环血浆和红细胞体积的短暂和可变减少，使准确估计血液中染料消失的速度变得困难。实验。手术可能会改变血清的固有颜色和染料消失的速度。
The application of a method for determining the plasma and total blood vol. employing the blue dye Evans Blue and the spectrophotometer to the investigation of clinical problems is described. Colorimetric errors inherent in earlier methods due to turbidity of plasma, lipemia, residual dye in repeated determinations and hemolysis of samples are minimized by the use of the spectrophotometer, and a spectrophotometric method of correcting for hemolysis is descr. Errors due to variations in dye mixing time ocqurring in different clinical states, and possible dilution of injected dye by lymph are eliminated by calculating the plasma vol. from a value obtained by extrapolation of the slope of disappearance of the dye from the blood stream, as detd. by multiple samples taken over a period of at least 30 min. after dye inj., to the time of inj. By the "direct" method of repeated single dets., vol. changes of clinical significance in the same individual can be reliably measured at frequent intervals. By the "indirect method" changes in vol. can be continuously followed for periods of from a few mins. to several hrs. Certain factors affecting the accuracy of the indirect method are discussed. A physiological response to serial blood sampling consisting of a transient and variable decrease in the circulating plasma and red cell vol. renders accurate estimation of the rate of disappearance of dye from the blood stream difficult. Exptl. procedures may alter the intrinsic color of the serum and rate of dye disappearance.