Capillary Coated with Graphene Oxide as Stationary Phase for the Separation of Brucine and Strychnine by Capillary Electrophoresis
Capillary Coated with Graphene Oxide as Stationary Phase for the Separation of Brucine and Strychnine by Capillary Electrophoresis
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DOI：
10.1093/chromsci/bmu076
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发表时间：
2015-04-01
影响因子：
1.3
通讯作者：
Ma, Jichao
中科院分区：
文献类型：
--
作者：
Li, Jian;Ye, Nengsheng;Ma, Jichao
以氧化石墨烯（GO）为固定相，建立了马钱子碱和士的宁的毛细管电泳分离新方法。考察了GO涂层毛细管对马钱子碱和士的宁的分离性能、重现性和稳定性。在优化分离条件后，选择含有25%（v/v）乙腈的磷酸盐溶液（40 mM，pH 7.0）作为运行缓冲液。与未涂层毛细管相比，由于分析物与毛细管固定相之间的相互作用增强，GO涂层毛细管具有更高的分离效率。马钱子碱和士的宁的线性范围为4.0-100.0 μ g·mL~（-1），相关系数R> 0.9994。该方法具有良好的分离效果和重现性。最后，该方法已成功地应用于中药制剂中这两种生物碱的测定。
A new capillary electrophoresis (CE) method was developed by using graphene oxide (GO) as a stationary phase for the separation of brucine and strychnine. The separation performance, reproducibility and stability of GO-coated capillary were investigated for the analysis of brucine and strychnine. After optimization of the separation conditions, a phosphate solution (40 mM, pH 7.0) containing 25% (v/v) acetonitrile was selected as the running buffer. Compared with uncoated capillary, higher separation efficiency was achieved by GO-coated capillary as a result of the increasing interactions between the analytes and the stationary phase of capillary. The linear ranges of these two alkaloids were 4.0-100.0 mu g mL(-1) with a satisfied correlation coefficients (R > 0.9994), and this novel method provided an efficient separation of brucine and strychnine as well as a good reproducibility and stability. Finally, the developed method was successfully applied for the determination of these two alkaloids in a pharmaceutical formulation of traditional Chinese medicines.