Detection of Circulating Tumor Cells by Fluorescence Microspheres-Mediated Amplification
Detection of Circulating Tumor Cells by Fluorescence Microspheres-Mediated Amplification
复制标题
通过荧光微球介导的扩增检测循环肿瘤细胞
DOI：
10.1021/acs.analchem.9b05844
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发表时间：
2020
影响因子：
7.4
通讯作者：
Jiang Xingyu
中科院分区：
文献类型：
--
作者：
Yin Jiaxiang;Deng Jinqi;Wang Le;Du Chang;Zhang Wei;Jiang Xingyu
在这里，我们描述了一种基于荧光微球的分离和分析方法，该方法能够从转移性癌症患者的全血中分离循环肿瘤细胞(CTCs)，并在不进行免疫染色的情况下原位鉴定分离的CTCs。这种方法使用抗体功能化的荧光聚苯乙烯(PS)微球，可以选择性地与CTCs结合。CTCs与荧光PS微球的结合形成了CTCs与荧光PS微球的复合体，从而实现了CTCs的尺寸放大和标记。采用微细加工技术制作了金字塔形微腔阵列(PMCA)，形成了高宽高比的精密微滤波结构。PMCA过滤装置可以有效地分离微球标记的CTCs，同时允许血细胞变形和通过。使用这种方法，在18例转移性结直肠癌患者中有15例分离和鉴定了CTC。这种方法将为CTCs的分离和鉴定开辟新的可能性，并可以为多种生物医学应用中的CTCs分析提供一个通用的平台。
Here we describe a fluorescent microspheres-based separation and analysis that enables the isolation of circulating tumor cells (CTCs) from whole blood of patients with metastatic cancer and the identification of isolated CTCs in situ without immunostaining. This approach uses antibody-functionalized fluorescent polystyrene (PS) microspheres that can selectively bind to CTCs. The binding of CTCs and fluorescent PS microspheres leads to the formation of complexes of CTCs and fluorescent PS microspheres, thereby the CTCs are size-amplified and labeled simultaneously. A pyramidal microcavity array (PMCA) is fabricated using microfabrication technology to create a precise microfilter structure with a high aspect ratio. The PMCA filter device can effectively isolate microspheres-labeled CTCs, while allow hematologic cells to deform and pass through. Using this approach, CTCs are isolated and identified in 15 of 18 patients with metastatic colorectal cancer. This approach will open new possibilities for CTCs isolation and identification and can serve a versatile platform to facilitate CTCs analysis in diverse biomedical applications.