Mechanically induced intracellular calcium waves in osteoblasts demonstrate calcium fingerprints in bone cell mechanotransduction
Mechanically induced intracellular calcium waves in osteoblasts demonstrate calcium fingerprints in bone cell mechanotransduction
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DOI：
10.1007/s10237-006-0059-5
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发表时间：
2007-11-01
影响因子：
3.5
通讯作者：
Donahue, Seth W.
中科院分区：
文献类型：
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作者：
Godin, Lindsay M.;Suzuki, Sakiko;Donahue, Seth W.
体外骨细胞机械刺激的早期反应是细胞内钙浓度 ([Ca2+](i)) 增加。本研究分析了两次相同的机械刺激（中间间隔 30 分钟的休息时间）的 [Ca2+](i) 波面积、幅度、持续时间、上升时间、下降时间和起始时间。与第一次加载相比，第二次加载回合中 [Ca2+](i) 波下的面积有所增加。这表明休息时间可能会增强机械诱导的细胞内钙信号。此外，许多 [Ca2+](i) 波参数在两次机械刺激之间呈强正相关。例如，在单个原代成骨细胞中，如果细胞在第一轮中具有较大的 [Ca2+](i) 波面积，则在第二轮中很可能具有较大的 [Ca2+](i) 波面积 (r(2) = 0.933)。这些发现支持这样的观点，即单个骨细胞具有“钙指纹”（即，独特的 [Ca2+](i) 波轮廓，可在重复暴露于给定刺激时重现）。
An early response to mechanical stimulation of bone cells in vitro is an increase in intracellular calcium concentration ([Ca2+](i)). This study analyzed the [Ca2+](i) wave area, magnitude, duration, rise time, fall time, and time to onset in individual osteoblasts for two identical bouts of mechanical stimulation separated by a 30-min rest period. The area under the [Ca2+](i) wave increased in the second loading bout compared to the first. This suggests that rest periods may potentiate mechanically induced intracellular calcium signals. Furthermore, many of the [Ca2+](i) wave parameters were strongly positively correlated between the two bouts of mechanical stimulation. For example, in individual primary osteoblasts, if a cell had a large [Ca2+](i) wave area in the first bout it was likely to have a large [Ca2+](i) wave area in the second bout (r(2) = 0.933). These findings support the idea that individual bone cells have "calcium fingerprints" (i.e., a unique [Ca2+](i) wave profile that is reproducible for repeated exposure to a given stimulus).