Comparison of Saccharomyces cerevisiae strains of clinical and nonclinical origin by molecular typing and determination of putative virulence traits
Comparison of Saccharomyces cerevisiae strains of clinical and nonclinical origin by molecular typing and determination of putative virulence traits
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DOI：
10.1111/j.1567-1364.2008.00365.x
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发表时间：
2008-06-01
影响因子：
3.2
通讯作者：
Jespersen, Lene
中科院分区：
文献类型：
--
作者：
Klingberg, Trine Dano;Lesnik, Urska;Jespersen, Lene
采用脉冲场凝胶电泳法对临床来源和非临床来源的酿酒酵母菌株进行了比较。尽管临床菌株和食品菌株有分别聚集的趋势，但临床来源的菌株和食品菌株之间的染色体长度多态并没有得到完全分离。除了一株食用菌外，所有的菌株都能在摄氏37度的温度下生长，但不能在42℃下生长。在假菌丝生长和侵袭力方面，菌株都有很大的变异，但这些特征与临床来源的菌株无关。测定了不同营养条件下酵母菌与人肠上皮细胞系(Caco-2)的黏附能力，以及对Caco-2细胞极化单层跨上皮电阻(TER)的影响。酵母菌对Caco-2细胞的黏附能力很低(0.6-6.2%)，临床菌株与非临床菌株之间无显著差异。临床和非临床来源的酿酒酵母均可增加Caco-2细胞极化单层的TER。根据本研究的结果，没有发现明确区分临床来源菌株和非临床来源菌株的特定毒力因子。相反，所有被调查的酿酒酵母菌株都被发现增强了上皮屏障功能。
Saccharomyces cerevisiae strains of clinical and nonclinical origin were compared by pulse field gel electrophoresis. Complete separation between strains of clinical origin and food strains by their chromosome length polymorphism was not obtained even though there was a tendency for the clinical and food strains to cluster separately. All the investigated strains, except for one food strain, were able to grow at temperatures >= 37 degrees C but not at 42 degrees C. Great strain variations were observed in pseudohyphal growth and invasiveness, but the characters were not linked to strains of clinical origin. The adhesion capacities of the yeast strains to a human intestinal epithelial cell line (Caco-2) in response to different nutritional availabilities were determined, as were the effects of the strains on the transepithelial electrical resistance (TER) across polarized monolayers of Caco-2 cells. The yeast strains displayed very low adhesion capacities to Caco-2 cells (0.6-6.2%), and no significant difference was observed between the strains of clinical and nonclinical origin. Both S. cerevisiae strains of clinical and non-clinical origin increased the TER of polarized monolayers of Caco-2 cells. Based on the results obtained in this study, no specific virulence factor was found that clearly separated the strains of clinical origin from the strains of nonclinical origin. On the contrary, all investigated strains of S. cerevisiae were found to strengthen the epithelial barrier function.