General Anesthesia Does Not Have Persistent Effects on Attention in Rodents
General Anesthesia Does Not Have Persistent Effects on Attention in Rodents
复制标题
DOI：
10.3389/fnbeh.2019.00076
复制
发表时间：
2019-04-17
影响因子：
3
通讯作者：
Mashour, George A.
中科院分区：
文献类型：
--
作者：
Hambrecht-Wiedbusch, Viviane S.;LaTendresse, Katherine A.;Mashour, George A.
背景资料：对动物的研究表明，全身麻醉可导致持续的空间记忆障碍，但麻醉剂对其他认知功能的影响尚不清楚。本研究测试是否暴露于全身麻醉而不手术引起持续赤字的注意力在啮齿动物。方法：为了评估麻醉是否有持续的影响，注意，大鼠随机分为三组。A组暴露于异氟醚麻醉2 h，并在随后的7天内测试注意力缺陷。B组为对照组，在注意力测试前接受室内空气。由于有一些证据表明亚麻醉剂量的氯胺酮可以改善认知并减少术后注意力障碍，因此在认知评估前，将C组大鼠暴露于异氟烷麻醉联合氯胺酮注射。使用5-Choice Serial Reaction Time Task测量大鼠的注意力，其中动物被训练在触摸屏上用鼻子戳来响应五个可能的网格位置之一的简短的不可预测的视觉刺激，以获得食物奖励。注意力进行了分析，%准确性，%遗漏，和过早respons.Results：评估急性注意力的基线值与干预后一天的数据进行比较，没有发现任何差异的注意力测量。没有显着差异被认为是在%的准确性，%遗漏，并为三组测试为连续7 days.Conclusion过早的反应：这些数据在健康的啮齿动物表明，全身麻醉没有手术没有持续的影响，注意力和氯胺酮的加入不会改变的结果。
Background: Studies in animals have shown that general anesthesia can cause persistent spatial memory impairment, but the influence of anesthetics on other cognitive functions is unclear. This study tested whether exposure to general anesthesia without surgery caused a persistent deficit in attention in rodents.Methods: To evaluate whether anesthesia has persistent effects on attention, rats were randomized to three groups. Group A was exposed for 2 h to isoflurane anesthesia, and tested the following seven days for attentional deficits. Group B was used as a control and received room air before attentional testing. Since there is some evidence that a subanesthetic dose of ketamine can improve cognition and reduce disorders of attention after surgery, rats in group C were exposed to isoflurane anesthesia in combination with a ketamine injection before cognitive assessment. Attention was measured in rats using the 5-Choice Serial Reaction Time Task, for which animals were trained to respond with a nose poke on a touchscreen to a brief, unpredictable visual stimulus in one of five possible grid locations to receive a food reward. Attention was analyzed as % accuracy, % omission, and premature responses.Results: Evaluating acute attention by comparing baseline values with data from the day after intervention did not reveal any differences in attentional measurements. No significant differences were seen in % accuracy, % omission, and premature responses for the three groups tested for 7 consecutive days.Conclusion: These data in healthy rodents suggest that general anesthesia without surgery has no persistent effect on attention and the addition of ketamine does not alter the outcome.