Telomere and 45S rDNA sequences are structurally linked on the chromosomes in Chrysanthemum segetum L.
Telomere and 45S rDNA sequences are structurally linked on the chromosomes in Chrysanthemum segetum L.
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端粒和 45S rDNA 序列在结构上连接在 Chrysanthemum segetum L. 的染色体上。
DOI：
10.1007/s00709-011-0279-0
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发表时间：
2011
期刊：
影响因子：
2.9
通讯作者：
Lijia Li
中科院分区：
文献类型：
--
作者：
Yong Hu;Jing Huang;Fei Yang;Jun Li;Lu Ma;Lu Zhang;Shibin He;Lijia Li
一些报道表明，核仁组织区位于端粒区，在许多生物体的细胞水平上与端粒具有结构联系。在这项研究中，我们发现所有45S核糖体DNA（rDNA）信号都位于菊花染色体上的端粒区域，并且45S rDNA在不同的中期染色体分布上显示出不同的信号模式。对延伸纤维的双色荧光原位杂交实验表明，端粒重复序列在结构上与rDNA序列连接或散布在其中。 rDNA和端粒序列之间密切的细胞学结构关系使得我们使用端粒引物和rDNA引物组合的PCR来获得一些片段，这些片段的两侧有不同的rDNA和端粒引物序列。一个代表性克隆CHS2含有紧密相连的rDNA和端粒序列，表明端粒序列侵入到保守的rDNA序列中。此外，一些PCR克隆的序列侧翼有单个端粒引物序列或rDNA引物序列。这些结果表明同源重组发生在rDNA序列的串联重复单元或染色体末端的端粒重复之间。
Some reports have shown that nucleolar organizer regions are located at the telomeric region and have a structural connection with telomeres at the cellular level in many organisms. In this study, we found that all 45S ribosomal DNA (rDNA) signals were located at telomeric regions on the chromosomes in Chrysanthemum segetum L., and the 45S rDNA showed distinct signal patterns on different metaphase chromosome spreads. The bicolor fluorescence in situ hybridization experiment on the extended fibers revealed that telomere repeats were structurally connected with or interspersed into rDNA sequences. The close cytological structure relation between rDNA and telomere sequences led us to use PCR with combinations of the telomere primer and the rDNA primer to obtain some fragments, which were flanked by different rDNA and telomere primer sequences. One representative clone CHS2 contains closely connected rDNA and telomere sequences, suggesting that the telomere sequence invaded into the conserved rDNA sequence. In addition, the sequences of some PCR clones were flanked by the single telomeric primer sequence or the rDNA primer sequence. These results suggested that homologous recombination occurred between tandem repeat units of rDNA sequences or telomere repeats at the chromosome terminus.
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