Development of polymorphic microsatellite markers in sesame (Sesamum indicum L.)
Development of polymorphic microsatellite markers in sesame (Sesamum indicum L.)
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DOI：
10.1111/j.1471-8286.2005.01048.x
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发表时间：
2005-12-01
期刊：
影响因子：
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通讯作者：
Cho, EG
中科院分区：
文献类型：
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作者：
Dixit, A;Jin, MH;Cho, EG
从芝麻（Sesamum indicum L.）使用修改后的协议。筛选出10个具有多态性的微卫星标记，对16份芝麻材料进行了多态性分析。每个位点的等位基因数为3 ~ 6个，平均4.6个。片段大小从150 bp到307 bp不等。预期杂合位点（H-E）和多态信息含量（PIC）的范围分别为0.437 ~ 0.858和0.34 ~ 0.80，表明微卫星的高信息量。这些微卫星标记将是非常有用的多样性分析之间的一个大的种质资源收集在我们的韩国基因库，也在建立其核心种质。
Fifty microsatellite sequences (SSRs) were isolated from an enriched library of sesame (Sesamum indicum L.) using a modified protocol. After screening, 10 polymorphic microsatellites were used to determine their usefulness in diversity analysis among 16 sesame accessions. The number of alleles ranged from three to six alleles per locus with an average of 4.6 alleles. The fragment size varied from 150 bp to 307 bp. Expected heterozygosites (H-E) and polymorphism information contents (PICs) ranged from 0.437 to 0.858 and 0.34 to 0.80, respectively, which indicates the highly informative nature of the microsatellites reported here. These microsatellite markers will be very useful in diversity analysis among a large germplasm collection of sesame present in our Korean gene bank and also in the establishment of its core collection.