Adipose differentiation-related protein: a gonadotropin- and prostaglandin-regulated protein in primate periovulatory follicles.
Adipose differentiation-related protein: a gonadotropin- and prostaglandin-regulated protein in primate periovulatory follicles.
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脂肪分化相关蛋白：灵长类排卵卵泡中的促性腺激素和前列腺素调节蛋白。
DOI：
10.1095/biolreprod.104.037523
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发表时间：
2005
影响因子：
3.6
通讯作者：
Duffy,DianeM
中科院分区：
文献类型：
--
作者：
Seachord,CarrieL;VandeVoort,CatherineA;Duffy,DianeM
周期中期LH峰刺激卵泡液中前列腺素E2（PGE 2）的升高，这是正常排卵所必需的。为了研究灵长类卵泡中PGE 2调节的过程，将猴颗粒细胞与单独的hCG或hCG和PGE 2一起培养，并将所得的总RNA进行微阵列分析。二十个PGE 2调节的mRNA被确定，我们选择了一个脂滴蛋白，脂肪分化相关蛋白（ADRP），为进一步研究。为了确定hCG和PGE 2是否在体内调节ADRP表达，猴子接受促性腺激素刺激多个卵泡发育。然后单独或与PG合成抑制剂塞来昔布联合给予人绒毛膜促性腺激素，并在40小时排卵期间隔内获得卵泡抽吸物或整个卵巢。hCG的管理增加颗粒细胞ADRP mRNA和蛋白质，在预期排卵时间之前测得峰值水平。与仅用hCG治疗的动物相比，hCG和塞来昔布治疗降低颗粒细胞ADRP mRNA水平。ADRP检测免疫细胞化学在许多猴组织中，合成阿利兰素，但不一致的类固醇合成组织表达。在hCG给药后，而非给药前，排卵期卵泡的颗粒细胞对ADRP进行免疫染色; ADRP与颗粒细胞质内的大脂滴共定位。这些研究确定ADRP作为一种新的促性腺激素和前列腺素E2调节蛋白在灵长类动物排卵期卵泡的颗粒细胞。由于ADRP促进非卵巢细胞的花生四烯酸摄取，ADRP相关的脂滴可能会增强颗粒细胞的花生四烯酸摄取，为排卵期前列腺素的产生提供前体。
The midcycle LH surge stimulates a rise in follicular fluid prostaglandin E2(PGE2), which is necessary for normal ovulation. To examine PGE2-regulated processes in primate follicles, monkey granulosa cells were cultured with hCG alone or with hCG and PGE2, and the resulting total RNA was subjected to microarray analysis. Twenty PGE2-regulated mRNAs were identified, and we selected a lipid droplet protein, adipose differentiation-related protein (ADRP), for further study. To determine whether hCG and PGE2regulate ADRP expression in vivo, monkeys received gonadotropins to stimulate multiple follicular development. Human chorionic gonadotropin was then administered alone or with the PG synthesis inhibitor celecoxib, and follicular aspirates or whole ovaries were obtained at times that span the 40-h periovulatory interval. Administration of hCG increased granulosa cell ADRP mRNA and protein, with peak levels measured just before the expected time of ovulation. Treatment with hCG and celecoxib decreased granulosa cellADRPmRNA levels compared with those of animals treated with hCG only. ADRP was detected by immunocytochemistry in many monkey tissues that synthesize prostaglandins but was not consistently expressed by steroidogenic tissues. Granulosa cells of periovulatory follicles immunostained for ADRP after, but not before, hCG administration; ADRP colocalized with large lipid droplets within the granulosa cell cytoplasm. These studies identify ADRP as a novel gonadotropin- and PGE2-regulated protein in the granulosa cells of primate periovulatory follicles. Because ADRP facilitates arachidonic acid uptake in nonovarian cells, ADRP-associated lipid droplets may enhance arachidonic acid uptake by granulosa cells to provide a precursor for periovulatory prostaglandin production.