HIV-1 Tat protein transduction domain peptide facilitates gene transfer in combination with cationic liposomes
HIV-1 Tat protein transduction domain peptide facilitates gene transfer in combination with cationic liposomes
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DOI：
10.1016/j.jconrel.2004.07.023
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发表时间：
2004-10-19
影响因子：
10.8
通讯作者：
Nan, XS
中科院分区：
文献类型：
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作者：
Hyndman, L;Lemoine, JL;Nan, XS
HIV-1 Tat蛋白的蛋白转导结构域（PTD）可以促进大分子颗粒的细胞和核摄取。在这里，我们证明，将 17 个氨基酸的 PTD 肽以非共价键的方式掺入基因递送脂复合物中可改善基因转移。通过绿色荧光蛋白表达质粒的荧光激活细胞扫描分析评估，Tat/脂质体/DNA (TLD) 转染能够以绵羊气管上皮的空气/液体界面培养物的形式转染高度顽抗的原代细胞。氯喹处理增加，低温孵育减少，TLD转染，提示内吞摄取途径参与。 (C) 2004 Elsevier B.V. 保留所有权利。
The protein transduction domain (PTD) of the HIV-1 Tat protein can facilitate the cellular and nuclear uptake of macromolecular particles. Here, we demonstrate that incorporation without covalent linkage of a 17-amino acid PTD peptide into gene delivery lipoplexes improves gene transfer. Tat/Liposome/DNA (TLD) transfection, as evaluated by Fluorescence Activated Cell Scan analysis of a Green Fluorescence Protein expression plasmid, enabled transfection of highly recalcitrant primary cells in the form of air/liquid interface cultures of sheep tracheal epithelium. Treatment with chloroquine increased, and incubation at low temperature decreased, TLD transfection, suggesting that the endocytosis uptake pathway is involved. (C) 2004 Elsevier B.V. All rights reserved.