Embryonated pigeon eggs as a model to investigate Neospora caninum infection
Embryonated pigeon eggs as a model to investigate Neospora caninum infection
复制标题
以鸽胚蛋为模型研究犬新孢子虫感染
DOI：
10.1177/0023677216652373
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发表时间：
2017
影响因子：
2.4
通讯作者：
S. Ghavami
中科院分区：
文献类型：
--
作者：
S. Bahrami;A. Rezaie;Z. Boroomand;M. Namavari;S. Ghavami
已有研究表明，鸡胚蛋可以作为实验性感染的动物模型。本研究的目的是探讨鸽胚蛋作为实验性新孢子病的动物模型。用犬新孢子虫Nc1株感染鸡、鸽胚蛋进行半数致死量(LD50)测定。计算LD50后，将2LD50的速殖子注射到虫卵中。观察每个胚胎的大体变化，并用免疫组织化学(IHC)和分子生物学方法研究寄生虫在组织中的分布。在本研究中，考虑了组织病理学的变化，并对用于组织病理学检查的组织切片，包括心脏、肝脏、脑和绒毛尿囊膜(CA)进行了IHC检查。鸽子胚胎比鸡胚表现出更多的宏观变化。大体病变以CA膜出血为主。显微镜检查发现急性新孢子症是由于出血、坏死和单个核炎性细胞的渗透所致。根据IHC和分子检测结果，在肝脏、心脏和CA膜上检测到寄生虫DNA。与鸡胚蛋一样，这些结果加强了鸽子胚蛋对犬新城疫的易感性，并为使用廉价和可用的动物模型进行犬新城疫研究提供了新的见解。本研究的结果表明，鸽子胚胎可能是研究犬新城疫活体生物学的一个很好的选择。
It has been shown that embryonated chicken eggs can be used as animal models for experimental infections. The aim of the present study was to investigate pigeon embryonated eggs as animal models for experimental neosporosis. An infection with Neospora caninum Nc1 isolate was conducted in chicken and pigeon embryonated eggs to evaluate LD50. After calculation of LD50, 2LD50 of tachyzoites were injected into the eggs. Macroscopic changes of each embryo were observed, and immunohistochemistry (IHC) and molecular methods were used to investigate the parasitic distribution in the tissues. In the present study, histopathological changes were considered, and sections of those used for histopathological examination including the heart, liver, brain and chorioallantoic (CA) membrane were also subjected to IHC. Pigeon embryos showed more macroscopic changes than chicken embryos. A hemorrhage of the CA membrane was the main gross lesion. Microscopic examination of tissues revealed acute neosporosis due to hemorrhage, necrosis and infiltration of mononuclear inflammatory cells. Based on IHC and molecular results, the parasite DNA was detected in the liver, heart and CA membrane. As with chicken embryonated eggs, these results reinforce the susceptibility of pigeon embryonated eggs to N. caninum, and provide new insights into using an inexpensive and available animal model for N. caninum research. The results of the present study suggest that pigeon embryos may be a good choice for studying the biology of N. caninum in living organisms.