New hair cells arise from supporting cell conversion in the acoustically damaged chick inner ear
New hair cells arise from supporting cell conversion in the acoustically damaged chick inner ear
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DOI：
10.1016/0304-3940(96)12367-3
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发表时间：
1996-02-16
影响因子：
2.5
通讯作者：
Raphael, Y
中科院分区：
文献类型：
--
作者：
Adler, HJ;Raphael, Y
支持细胞有丝分裂对受声损伤的鸟类内耳毛细胞再生有显着贡献。然而，毛细胞替代可能还有另一种机制：支持细胞转化。本研究使用 DNA 合成抑制剂阿糖胞苷 (Ara-C) 来更好地确定支持细胞是否可以在不进行细胞分裂的情况下转分化为毛细胞。小鸡在过度声刺激后接受阿糖胞苷注射。对基底乳头的扫描显微镜研究揭示了一些未配对的、未成熟的毛细胞。为了确保 Ara-C 阻断 DNA 合成，一组鸟类同时接受 Ara-C 和溴脱氧尿苷 (BrdU)，而另一组仅接受 BrdU。 Ara-C/BrdU 和 BrdU 乳头的免疫细胞化学分析分别表明有 0 个和 16 个分裂细胞。这种差异证实了 Ara-C 阻断了 DNA 合成，阻止了支持细胞的有丝分裂。这些数据强烈表明支持细胞可以转化为毛细胞。
Supporting cell mitosis contributes significantly to hair cell regeneration in the acoustically damaged bird inner ear. Yet there may be another mechanism of hair cell replacement: supporting cell conversion. This study used cytosine arabinoside (Ara-C), an inhibitor of DNA synthesis, to better determine whether supporting cells could transdifferentiate into hair cells without cell division. Chicks received Ara-C injections after acoustic overstimulation. Scanning microscopic studies of the basilar papillae revealed several unpaired, immature hair cells. To ensure Ara-C's blockage of DNA synthesis, one group of birds received both Ara-C and bromodeoxyuridine (BrdU), while another group had BrdU only. Immunocytochemical analysis of Ara-C/BrdU and BrdU papillae indicated zero and 16 dividing cells, respectively. This difference confirmed that Ara-C blocked DNA synthesis, arresting supporting cell mitosis. These data strongly suggest that supporting cells can convert into hair cells.