WHOLE GENOME AMPLIFICATION FROM A SINGLE CELL - IMPLICATIONS FOR GENETIC-ANALYSIS
WHOLE GENOME AMPLIFICATION FROM A SINGLE CELL - IMPLICATIONS FOR GENETIC-ANALYSIS
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DOI：
10.1073/pnas.89.13.5847
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发表时间：
1992-07-01
影响因子：
11.1
通讯作者：
ARNHEIM, N
中科院分区：
文献类型：
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作者：
ZHANG, L;CUI, XF;ARNHEIM, N
我们开发了一种体外方法，通过使用15个碱基的随机寡核苷酸混合物进行重复的引物延伸，来扩增单倍体细胞中存在的大部分DNA序列。我们研究了12个基因位点，并估计将基因组中任何序列扩增到至少30个拷贝的概率不低于0.78（95%置信度）。从单个细胞或其他含极少量DNA的样本开始的全基因组扩增，对通过精子或卵母细胞分型进行多点定位，以及可能对遗传病诊断、法医学和古代DNA样本分析都具有重要意义。
We have developed an in vitro method for amplifying a large fraction of the DNA sequences present in a single haploid cell by repeated primer extensions using a mixture of 15-base random oligonucleotides. We studied 12 genetic loci and estimate that the probability of amplifying any sequence in the genome to a minimum of 30 copies is not less than 0.78 (95% confidence). Whole genome amplification beginning with a single cell, or other samples with very small amounts of DNA, has significant implications for multipoint mapping by sperm or oocyte typing and possibly for genetic disease diagnosis, forensics, and the analysis of ancient DNA samples.