Effects of tear substitutes on conjunctival epithelium of mice
Effects of tear substitutes on conjunctival epithelium of mice
复制标题
DOI：
10.1159/000108120
复制
发表时间：
2007-01-01
影响因子：
2.1
通讯作者：
Puzzolo, Domenico
中科院分区：
文献类型：
--
作者：
Aragona, Pasquale;Micali, Antonio;Puzzolo, Domenico
目的：研究了给予三种泪液替代物对小鼠正常结膜上皮的影响，特别是对杯状细胞的影响。方法：将3月龄Swiss CD 1小鼠分为4组，每组7只。第1组为未处理组（对照组）。其他动物以5滴/天滴注，持续10天，如下所示：第2组，0.2%透明质酸钠（SH）;第3组，0.5%羟丙基甲基纤维素（HPMC）;第4组，0.9% NaCl。对整个结膜进行光学和电子显微镜检查。进行形态计量学研究以评价：杯状细胞的数量和大小以及粘蛋白颗粒的数量、大小和光密度。此外，进行杯状细胞的阿辛蓝/高碘酸-希夫（AB/ PAS）染色。结果如下：SH处理组杯状细胞的面积和数量与未处理组相似，粘蛋白颗粒的数量和光密度减少，但尺寸增加。HPMC处理组显示杯状细胞面积减少，粘蛋白颗粒光密度降低。生理盐水处理组杯状细胞面积和数量减少，粘蛋白颗粒数量和光密度减少，大小增加。AB/PAS染色显示，每种处理都能够诱导杯状细胞染色特征的处理特异性修饰。结论：我们的研究结果强烈表明，泪液代用品可以诱导结膜上皮细胞的治疗特异性修改，特别是关于杯状细胞结构和超微结构。
Aim: The effects of the administration of three tear substitutes on normal conjunctival epithelium of the mouse, with particular regard to goblet cells, were studied. Methods: Three-month-old Swiss CD 1 mice were divided into four groups of 7 animals each. Group 1 was untreated (control). The other animals were treated with the instillation of 5 drops/day for 10 days as follows: group 2, 0.2% sodium hyaluronate (SH); group 3, 0.5% hydroxypropylmethylcellulose (HPMC); group 4, 0.9% NaCl. The entire conjunctiva was processed for light and electron microscopy. A morphometric study was performed to evaluate: the number and size of goblet cells and the number, size and optical density of mucin granules. Furthermore, Alcian blue/periodic acid-Schiff (AB/ PAS) staining for goblet cells was carried out. Results: The SH-treated group showed that the area and number of goblet cells were similar to the untreated group; the mucin granules were reduced in number and optical density but increased in size. The HPMC-treated group showed a reduced area of goblet cells with reduced optical density of mucin granules. The saline- treated group showed that goblet cells were reduced in area and number; mucin granules showed a reduction in number and optical density and an increased size. The AB/PAS stain showed that each treatment was able to induce a treatment-specific modification of the staining characteristics of goblet cells. Conclusion: Our results strongly suggest that tear substitutes can induce treatment-specific modifications of the conjunctival epithelium with particular regard to goblet cell structure and ultrastructure.